AB300584

Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CTRP6 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

View Alternative Names

CTRP6, UNQ581/PRO1151, C1QTNF6, Complement C1q tumor necrosis factor-related protein 6

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Supplier Data

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free) (AB300584)

This data was developed using ab300583, the same antibody clone in a different buffer formulation.

CTRP6 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab300583 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300583 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

All lanes:

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (<a href='/en-us/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a>) at 1/30 dilution

Lane 1:

Human placenta tissue lysate (Inset) at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a> IP in Human placenta tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a> in human placenta tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free) (AB300584)

This data was developed using ab300583, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 28726640)

All lanes:

Western blot - Anti-CTRP6 antibody [EPR25750-35] (<a href='/en-us/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a>) at 1/1000 dilution

Lane 1:

human placenta tissue lysate 40 µg

Lane 2:

human skeletal muscle tissue lysate 40 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free) (AB300584)

This data was developed using ab300583, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human CTRP8.

All lanes:

Western blot - Anti-CTRP6 antibody [EPR25750-35] (<a href='/en-us/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a>) at 1/1000 dilution

Lane 1:

Human CTRP6 recombinant protein 5 ng

Lane 2:

His-tagged human CTRP8 recombinant protein 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3.25s

Unconjugated

Anti-CTRP6 antibody [EPR25750-35]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25750-35

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTRP6 also known as C1q/TNF-related protein 6 is a secreted protein that belongs to the C1q/TNF-related protein family. It has a molecular mass of approximately 37 kDa. Expression of CTRP6 occurs in various tissues including adipose tissue skeletal muscle and liver. Its structural components include a collagen-like domain and a C1q globular domain which are characteristic of proteins involved in immune responses and metabolic regulation.

Biological function summary

CTRP6 plays a significant role in modulating inflammatory responses and metabolism. It acts by influencing the secretion and activity of cytokines such as IL-6 and TNF-alpha. Although not frequently noted as part of a multi-protein complex CTRP6 interacts closely with signaling molecules to achieve its functions. Studies suggest its involvement in maintaining metabolic balance and regulating insulin sensitivity key processes in various physiological states.

Pathways

It interacts with several metabolic and inflammatory signaling cascades. This protein mediates effects through pathways like the AMPK signaling pathway which plays an important role in energy balance and metabolic stress responses. CTRP6 is also linked to the TLR4 signaling pathway involved in immune activation and inflammation. These pathways involve interactions with various proteins such as AMPK and TLR4 receptors highlighting its integrative role in cell signaling networks.

CTRP6 shows an association with conditions like obesity and type 2 diabetes. These diseases link to its role in regulating metabolic and inflammatory responses. Dysfunctional CTRP6 expression or activity has been observed in individuals with these metabolic disorders. Additionally CTRP6 relates to the expression and regulation of proteins such as adiponectin and TNF-alpha which are also involved in these disease pathways. These connections emphasize its potential as a therapeutic target for metabolic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information C1QTNF6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free)

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free) (AB300584)

Western blot - Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free) (AB300584)

Western blot - Anti-CTRP6 antibody [EPR25750-35] (BSA and Azide free) (AB300584)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise