Rabbit Recombinant Monoclonal Cubilin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (AB251050), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-bsa-and-azide-free-ab251050--western-blot-img83752.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (AB251050)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (AB251050), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-bsa-and-azide-free-ab251050--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123948.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (AB251050)")
Publications
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- Experimental and therapeutic medicine 25:842023Effect of nicotine on cholesterol gallstone formation in C57BL/6J mice fed on a lithogenic diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Gao,Pinduan Bi,Qili Mi,Ying Guan,Jiarui Jiang,Xuemei Li,Bin YangPubMed 36684657
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake (PubMed:10371504, PubMed:11606717, PubMed:11717447, PubMed:14576052, PubMed:9572993). Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex (PubMed:14576052, PubMed:9572993). Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium (PubMed:9572993). Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
Alternative names
IFCR, CUBN, Cubilin, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-cobalamin receptor, Intrinsic factor-vitamin B12 receptor
Recommended products
Rabbit Recombinant Monoclonal Cubilin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16259-27
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251050 is the carrier-free version of Anti-Cubilin antibody [EPR16259-27] - C-terminal ab191073.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (ab251050), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-bsa-and-azide-free-ab251050--western-blot-img83752.jpg "Western blot - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (ab251050)")
Western blot - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (ab251050)
This data was developed using Anti-Cubilin antibody [EPR16259-27] - C-terminal ab191073, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (Anti-Cubilin antibody [EPR16259-27] - C-terminal ab191073) at 1/50000 dilution
All lanes: Human fetal kidney tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 399 kDa
Observed band size: 399 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (ab251050), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-bsa-and-azide-free-ab251050--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123948.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (ab251050)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - BSA and Azide free (ab251050)
This data was developed using Anti-Cubilin antibody [EPR16259-27] - C-terminal ab191073, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Cubilin with Anti-Cubilin antibody [EPR16259-27] - C-terminal ab191073 at a 1/500 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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