Rabbit Recombinant Monoclonal Cubilin antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (AB191073), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-c-terminal-ab191073--western-blot-img83752.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (AB191073)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - C-terminal (AB191073), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-c-terminal-ab191073--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123948.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - C-terminal (AB191073)")
Publications
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- Aging 13:18340-183592021CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunfen Xu,Weiguo LuPubMed 34326275
- PLoS pathogens 16:e10090532020A vector whitefly endocytic receptor facilitates the entry of begomoviruses into its midgut cells via binding to virion capsid proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhao et. al.PubMed 33270808
- International journal of molecular sciences 20:2019iPSC-Derived Embryoid Bodies as Models of c--Mutated Hereditary Papillary Renal Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Wook Hwang et. al.PubMed 31575031
- Diabetes, metabolic syndrome and obesity : targets 12:1583-15962019The effect of A1 adenosine receptor in diabetic megalin loss with caspase-1/IL18 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongli Tian et. al.PubMed 31695457
- Journal of diabetes investigation 10:613-6252018Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiqin Yin,Yongwei Jiang,Shiqing Xu,Zai Wang,Liang Peng,Qing Fang,Tingting Deng,Wanni Zhao,Wenjian Zhang,Jinning LouPubMed 30307132
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake (PubMed:10371504, PubMed:11606717, PubMed:11717447, PubMed:14576052, PubMed:9572993). Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex (PubMed:14576052, PubMed:9572993). Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium (PubMed:9572993). Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
Alternative names
IFCR, CUBN, Cubilin, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-cobalamin receptor, Intrinsic factor-vitamin B12 receptor
Recommended products
Rabbit Recombinant Monoclonal Cubilin antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR16259-27
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-c-terminal-ab191073--western-blot-img83752.jpg "Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073)")
Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073)
All lanes: Western blot - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073) at 1/50000 dilution
All lanes: Human fetal kidney tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 399 kDa
Observed band size: 399 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073), expandable thumbnail](https://content.abcam.com/products/images/cubilin-antibody-epr16259-27-c-terminal-ab191073--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123948.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cubilin antibody [EPR16259-27] - C-terminal (ab191073)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Cubilin with ab191073 at a 1/500 dilution.
Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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