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AB129115

Anti-CUG-BP1 antibody [EPR8298(B)]

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(13 Publications)

Rabbit Recombinant Monoclonal CUG-BP1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

BRUNOL2, CUGBP, CUGBP1, NAB50, CELF1, CUGBP Elav-like family member 1, CELF-1, 50 kDa nuclear polyadenylated RNA-binding protein, Bruno-like protein 2, CUG triplet repeat RNA-binding protein 1, CUG-BP- and ETR-3-like factor 1, Deadenylation factor CUG-BP, Embryo deadenylation element-binding protein homolog, RNA-binding protein BRUNOL-2, CUG-BP1, EDEN-BP homolog

3 Images
Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (AB129115)
  • WB

Unknown

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (AB129115)

All lanes:

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

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Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (AB129115)
  • WB

Lab

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (AB129115)

Lanes 1-4 : Merged signal (red and green). Green - ab129115 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129115 Anti-CUG-BP1 antibody [EPR8298(B)] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab129115 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CELF1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CELF1 (CUG-BP1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-celf1-cug-bp1-knockout-hek-293t-cell-line-ab266086'>ab266086</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (AB129115)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] (AB129115)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8298(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CUG-BP1 also known as CELF1 belongs to the CELF family of RNA-binding proteins. It is a 52-kDa protein expressed across various tissues with noticeable levels in heart and skeletal muscle. CUG-BP1 regulates alternative splicing by binding to specific CUG triplet repeats in RNA molecules. This binding influences the processing of pre-mRNAs impacting gene expression at the post-transcriptional level.
Biological function summary

The regulation by CUG-BP1 affects both mRNA stability and translation. The protein plays an important role in managing the expression of genes involved in muscle differentiation and development. CUG-BP1 often interacts with other cellular machinery forming functional complexes to facilitate its role in RNA metabolism. Its capacity to bind CUG motifs on target RNA highlights its significance in cellular processes.

Pathways

CUG-BP1 participates in pathways influencing muscle formation and maintenance. It plays part in the regulation of alternative splicing processes critical for efficient functioning of the p38 MAPK pathway. This pathway connects CUG-BP1 to other RNA-binding proteins and kinases. By doing so it affects different transcriptional and post-transcriptional processes emphasizing its critical function in cellular maintenance.

CUG-BP1 has been linked to myotonic dystrophy type 1 (DM1) and certain cancers. In DM1 the protein becomes sequestered by expanded CUG repeats in RNA disrupting its normal function. This sequestration leads to misregulated splicing events affecting various cellular functions. CUG-BP1’s interaction with other proteins such as MBNL1 further elucidates its role in disease pathogenesis highlighting its importance in understanding RNA-mediated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts both as an activator and as a repressor of a pair of coregulated exons : promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA (PubMed : 28431233).
See full target information CELF1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7414 PubMed40789858

2025

Adipocyte RNA-binding protein CELF1 promotes beiging of white fat through stabilizing Dio2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zeng,Liuling Xiao,Jiajie Li,Han Wu,Xiaolong Guo,Fukang Zhu,Xinyu Yu,Yewei Cui,Xueya Zhao,Yumeng Wang,Ting Zhang,Weijiong He,Hongxiang Zeng,Xi Li

Frontiers in pharmacology 14:1183720 PubMed37731742

2023

The multiple functions of miR-574-5p in the neuroblastoma tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Proestler,Julia Donzelli,Sheila Nevermann,Kai Breitwieser,Leon F Koch,Tatjana Best,Maria Fauth,Malin Wickström,Patrick N Harter,Per Kogner,Grégory Lavieu,Karin Larsson,Meike J Saul

Cells 12: PubMed36672166

2023

Early Regional Patterning in the Human Prefrontal Cortex Revealed by Laminar Dynamics of Deep Projection Neuron Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Janja Kopić,Alisa Junaković,Iva Salamon,Mladen-Roko Rasin,Ivica Kostović,Željka Krsnik

Disease models & mechanisms 15: PubMed35642886

2022

Condensation properties of stress granules and processing bodies are compromised in myotonic dystrophy type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Selma Gulyurtlu,Monika S Magon,Patrick Guest,Panagiotis P Papavasiliou,Kim D Morrison,Alan R Prescott,Judith E Sleeman

Cell 184:6174-6192.e32 PubMed34813726

2021

Xist nucleates local protein gradients to propagate silencing across the X chromosome.

Applications

Unspecified application

Species

Unspecified reactive species

Yolanda Markaki,Johnny Gan Chong,Yuying Wang,Elsie C Jacobson,Christy Luong,Shawn Y X Tan,Joanna W Jachowicz,Mackenzie Strehle,Davide Maestrini,Abhik K Banerjee,Bhaven A Mistry,Iris Dror,Francois Dossin,Johannes Schöneberg,Edith Heard,Mitchell Guttman,Tom Chou,Kathrin Plath

Journal of extracellular vesicles 10:e12143 PubMed34596365

2021

Small extracellular vesicle-derived miR-574-5p regulates PGE2-biosynthesis via TLR7/8 in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Donzelli,Eva Proestler,Anna Riedel,Sheila Nevermann,Brigitte Hertel,Andreas Guenther,Stefan Gattenlöhner,Rajkumar Savai,Karin Larsson,Meike J Saul

Bioscience reports 41: PubMed33973628

2021

Potential role for pyruvate kinase M2 in the regulation of murine cardiac glycolytic flux during in vivo chronic hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Michal K Handzlik,David J Tooth,Dumitru Constantin-Teodosiu,Paul L Greenhaff,Mark A Cole

Nature 587:145-151 PubMed32908311

2020

A protein assembly mediates Xist localization and gene silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Amy Pandya-Jones,Yolanda Markaki,Jacques Serizay,Tsotne Chitiashvili,Walter R Mancia Leon,Andrey Damianov,Constantinos Chronis,Bernadett Papp,Chun-Kan Chen,Robin McKee,Xiao-Jun Wang,Anthony Chau,Shan Sabri,Heinrich Leonhardt,Sika Zheng,Mitchell Guttman,Douglas L Black,Kathrin Plath

Frontiers in pharmacology 11:196 PubMed32231562

2020

Proteomics-Based Characterization of miR-574-5p Decoy to CUGBP1 Suggests Specificity for mPGES-1 Regulation in Human Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anne C Emmerich,Julia Wellstein,Elena Ossipova,Isabell Baumann,Johan Lengqvist,Kim Kultima,Per-Johan Jakobsson,Dieter Steinhilber,Meike J Saul

FASEB journal : official publication of the Federa 33:6933-6947 PubMed30922080

2019

miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction.

Applications

Unspecified application

Species

Unspecified reactive species

Meike J Saul,Isabell Baumann,Annalisa Bruno,Anne C Emmerich,Julia Wellstein,Sarah M Ottinger,Annalisa Contursi,Melania Dovizio,Sandra Donnini,Stefania Tacconelli,Joan Raouf,Helena Idborg,Stefan Stein,Marina Korotkova,Rajkumar Savai,Erika Terzuoli,Gianluca Sala,Werner Seeger,Per-Johan Jakobsson,Paola Patrignani,Beatrix Suess,Dieter Steinhilber
View all publications
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