AB240044

Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal CUG-BP1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BRUNOL2, CUGBP, CUGBP1, NAB50, CELF1, CUGBP Elav-like family member 1, CELF-1, 50 kDa nuclear polyadenylated RNA-binding protein, Bruno-like protein 2, CUG triplet repeat RNA-binding protein 1, CUG-BP- and ETR-3-like factor 1, Deadenylation factor CUG-BP, Embryo deadenylation element-binding protein homolog, RNA-binding protein BRUNOL-2, CUG-BP1, EDEN-BP homolog

2 Images

Lab

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free (AB240044)

This data was developed using the same antibody clone in a different buffer formulation (ab129115).

Lanes 1-4 : Merged signal (red and green). Green - ab129115 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129115 Anti-CUG-BP1 antibody [EPR8298(B)] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab129115 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] (<a href='/en-us/products/primary-antibodies/cug-bp1-antibody-epr8298b-ab129115'>ab129115</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CELF1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CELF1 (CUG-BP1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-celf1-cug-bp1-knockout-hek-293t-cell-line-ab266086'>ab266086</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free (AB240044)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free (AB240044)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-CUG-BP1 antibody [EPR8298(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CUG-BP1 antibody [EPR8298(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CUG-BP1 antibody [EPR8298(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8298(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab240044 is the carrier-free version of ab129115.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CUG-BP1 also known as CELF1 belongs to the CELF family of RNA-binding proteins. It is a 52-kDa protein expressed across various tissues with noticeable levels in heart and skeletal muscle. CUG-BP1 regulates alternative splicing by binding to specific CUG triplet repeats in RNA molecules. This binding influences the processing of pre-mRNAs impacting gene expression at the post-transcriptional level.

Biological function summary

The regulation by CUG-BP1 affects both mRNA stability and translation. The protein plays an important role in managing the expression of genes involved in muscle differentiation and development. CUG-BP1 often interacts with other cellular machinery forming functional complexes to facilitate its role in RNA metabolism. Its capacity to bind CUG motifs on target RNA highlights its significance in cellular processes.

Pathways

CUG-BP1 participates in pathways influencing muscle formation and maintenance. It plays part in the regulation of alternative splicing processes critical for efficient functioning of the p38 MAPK pathway. This pathway connects CUG-BP1 to other RNA-binding proteins and kinases. By doing so it affects different transcriptional and post-transcriptional processes emphasizing its critical function in cellular maintenance.

CUG-BP1 has been linked to myotonic dystrophy type 1 (DM1) and certain cancers. In DM1 the protein becomes sequestered by expanded CUG repeats in RNA disrupting its normal function. This sequestration leads to misregulated splicing events affecting various cellular functions. CUG-BP1’s interaction with other proteins such as MBNL1 further elucidates its role in disease pathogenesis highlighting its importance in understanding RNA-mediated disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts both as an activator and as a repressor of a pair of coregulated exons : promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA (PubMed : 28431233).

See full target information CELF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 9:1055356 PubMed36518851

2022

The copper transporter CTR1 and cisplatin accumulation at the single-cell level by LA-ICP-TOFMS.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Schoeberl,Michael Gutmann,Sarah Theiner,Mario Corte-Rodríguez,Gabriel Braun,Petra Vician,Walter Berger,Gunda Koellensperger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free

Western blot - Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free (AB240044)

OI-RD Scanning - Anti-CUG-BP1 antibody [EPR8298(B)] - BSA and Azide free (AB240044)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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