AB76470

Anti-CUL-4A + CUL-4B antibody [EPR3200]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal Cullin 4B/CUL-4B antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

KIAA0695, CUL4B, Cullin-4B, CUL-4B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (AB76470)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (AB76470)

ab76470 at 1/100 dilution staining Cullin 4B in human brain tissue using a HRP/AP polymerized secondary antibody.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (AB76470)

All lanes:

Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470) at 1/5000 dilution

Lane 1:

Fetal uterus lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

A375 lysate at 10 µg

Lane 4:

Fetal testis lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

false

Carrier free

Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3200

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CUL-4A and CUL-4B also known as cullin-4A and cullin-4B are part of the cullin protein family integral to protein degradation processes. These proteins form a scaffold for E3 ubiquitin ligases facilitating the transfer of ubiquitin to substrate proteins marking them for proteasomal degradation. CUL-4A is approximately 87 kDa while CUL-4B is about 104 kDa. Expression is seen in various tissues including testes placenta and brain with CUL-4B having a distinct role in neuronal tissues.

Biological function summary

CUL-4A and CUL-4B contribute significantly to cell cycle regulation and DNA damage response. They are part of the CUL4-DDB1 E3 ubiquitin-protein ligase complexes which handle the ubiquitination of various substrates involved in cell cycle progression and genome stability. These complexes help maintain chromatin structure and secure the genome's integrity by influencing the replication and repair processes ensuring accurate transmission of genetic material.

Pathways

CUL-4A and CUL-4B are significant players in the DNA damage repair and cell cycle control pathways. They collaborate with proteins like DDB1 (damage-specific DNA binding protein 1) and CDT1 (chromatin licensing and DNA replication factor 1) to modulate cell cycle checkpoints and repair mechanisms. Their activity impacts the ATR/ATM pathways ensuring that any DNA damage is effectively managed to prevent genomic instability which could lead to various diseases.

Aberrations in CUL-4A and CUL-4B function have links to cancer and neurological disorders. Changes in their activity can lead to unchecked cell proliferation contributing to tumorigenesis in cancers such as breast cancer. In neurological disorders particularly those affecting brain development abnormal expression of CUL-4B is notably impactful interacting with proteins like DDB1 in both disease progression and development. These associations highlight their importance as potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14578910, PubMed : 16322693, PubMed : 16678110, PubMed : 18593899, PubMed : 22118460, PubMed : 29779948, PubMed : 30166453, PubMed : 33854232, PubMed : 33854239). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit (PubMed : 14578910, PubMed : 16678110, PubMed : 18593899, PubMed : 22118460, PubMed : 29779948). CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed : 14578910, PubMed : 16678110, PubMed : 18593899, PubMed : 22118460). Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage (PubMed : 14578910, PubMed : 16678110, PubMed : 18593899). Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication (PubMed : 16678110). A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed : 29779948). The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3) (PubMed : 33854232, PubMed : 33854239). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed : 30166453). Required for ubiquitination of cyclin E (CCNE1 or CCNE2), and consequently, normal G1 cell cycle progression (PubMed : 16322693, PubMed : 19801544). Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism (PubMed : 18235224). Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (PubMed : 18235224). With CUL4A, contributes to ribosome biogenesis (PubMed : 26711351).

See full target information CUL4B

Additional targets

Cullin-4A,CUL4B

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402066 PubMed38940381

2024

Uremic Toxin Receptor AhR Facilitates Renal Senescence and Fibrosis via Suppressing Mitochondrial Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Xie,Ninghao Yang,Li Lu,Xi'ang Sun,Jingyao Li,Xin Wang,Hengjiang Guo,Li Zhou,Jun Liu,Huijuan Wu,Chen Yu,Wei Zhang,Limin Lu

Journal of biochemical and molecular toxicology 37:e23383 PubMed37158446

2023

Knockdown of circMFN2 inhibits cell progression and glycolysis by miR-198/CUL4B pathway in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Song,Ting Chai,Junqi Liu,Alan Chu,Chen Sun,Zongwen Liu

Bioengineered 12:4719-4735 PubMed34338146

2021

Inhibition of MicroRNA miR-101-3p on prostate cancer progression by regulating Cullin 4B (CUL4B) and PI3K/AKT/mTOR signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Gu,Zhixin You,Yucheng Yang,Rui Ding,Meili Wang,Jianming Pu,Jian Chen

The Journal of biological chemistry 295:3148-3158 PubMed32014991

2020

Genome-wide siRNA screening reveals that DCAF4-mediated ubiquitination of optineurin stimulates autophagic degradation of Cu,Zn-superoxide dismutase.

Applications

Unspecified application

Species

Unspecified reactive species

Kengo Homma,Hiromitsu Takahashi,Naomi Tsuburaya,Isao Naguro,Takao Fujisawa,Hidenori Ichijo

Cell death and differentiation 27:984-998 PubMed31367012

2019

CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Maria G Füzesi-Levi,Irit Fainer,Radoslav Ivanov Enchev,Gili Ben-Nissan,Yishai Levin,Meital Kupervaser,Gilgi Friedlander,Tomer Meir Salame,Reinat Nevo,Matthias Peter,Michal Sharon

Science advances 5:eaau7130 PubMed30775435

2019

Restriction of hepatitis B virus replication by c-Abl-induced proteasomal degradation of the viral polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Lidan Hou,Jie Zhao,Shaobing Gao,Tong Ji,Tianyu Song,Yining Li,Jingjie Wang,Chenlu Geng,Min Long,Jiang Chen,Hui Lin,Xiujun Cai,Yong Cang

PLoS genetics 14:e1007165 PubMed29370161

2018

CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Song,Shenghui Liang,Jiye Liu,Tingyue Zhang,Yifei Yin,Chenlu Geng,Shaobing Gao,Yan Feng,Hao Xu,Dongqing Guo,Amanda Roberts,Yuchun Gu,Yong Cang

Biology of reproduction 94:65 PubMed26864195

2016

The E3 Ubiquitin Ligase CRL4 Regulates Proliferation and Progression Through Meiosis in Chinese Mitten Crab Eriocheir sinensis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Li Wang,Dan Li,Hong-Dan Yang,Lin He,Wen-Juan Sun,Ze-Lin Duan,Qun Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CUL-4A + CUL-4B antibody [EPR3200]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (AB76470)

Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (AB76470)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (8)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

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