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AB247477

Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Cullin 4A/CUL-4A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Cullin-4A, CUL-4A, CUL4A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (AB247477)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (AB247477)

This data was developed using ab76470, the same antibody clone in a different buffer formulation.

ab76470 at 1/100 dilution staining Cullin 4B in human brain tissue, using a HRP/AP polymerized secondary antibody.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (AB247477)
  • WB

Unknown

Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] - BSA and Azide free (AB247477)

This data was developed using ab76470, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (<a href='/en-us/products/primary-antibodies/cul-4a-cul-4b-antibody-epr3200-ab76470'>ab76470</a>) at 1/5000 dilution

Lane 1:

Fetal uterus lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

A375 lysate at 10 µg

Lane 4:

Fetal testis lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3200

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247477 is the carrier-free version of ab76470.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CUL-4A and CUL-4B also known as cullin-4A and cullin-4B are part of the cullin protein family integral to protein degradation processes. These proteins form a scaffold for E3 ubiquitin ligases facilitating the transfer of ubiquitin to substrate proteins marking them for proteasomal degradation. CUL-4A is approximately 87 kDa while CUL-4B is about 104 kDa. Expression is seen in various tissues including testes placenta and brain with CUL-4B having a distinct role in neuronal tissues.
Biological function summary

CUL-4A and CUL-4B contribute significantly to cell cycle regulation and DNA damage response. They are part of the CUL4-DDB1 E3 ubiquitin-protein ligase complexes which handle the ubiquitination of various substrates involved in cell cycle progression and genome stability. These complexes help maintain chromatin structure and secure the genome's integrity by influencing the replication and repair processes ensuring accurate transmission of genetic material.

Pathways

CUL-4A and CUL-4B are significant players in the DNA damage repair and cell cycle control pathways. They collaborate with proteins like DDB1 (damage-specific DNA binding protein 1) and CDT1 (chromatin licensing and DNA replication factor 1) to modulate cell cycle checkpoints and repair mechanisms. Their activity impacts the ATR/ATM pathways ensuring that any DNA damage is effectively managed to prevent genomic instability which could lead to various diseases.

Aberrations in CUL-4A and CUL-4B function have links to cancer and neurological disorders. Changes in their activity can lead to unchecked cell proliferation contributing to tumorigenesis in cancers such as breast cancer. In neurological disorders particularly those affecting brain development abnormal expression of CUL-4B is notably impactful interacting with proteins like DDB1 in both disease progression and development. These associations highlight their importance as potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620, PubMed : 30166453, PubMed : 33854232, PubMed : 33854239). As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). DCX(DET1-COP1) directs ubiquitination of JUN (PubMed : 14739464). DCX(DDB2) directs ubiquitination of XPC (PubMed : 15811626). DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition (PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53/TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed : 14578910, PubMed : 15448697, PubMed : 15548678). DCX(DTL) directs autoubiquitination of DTL (PubMed : 23478445). In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip (PubMed : 16537899). Is involved in ubiquitination of HOXA9 (PubMed : 14609952). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed : 26431207). A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed : 29779948). The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3) (PubMed : 33854232, PubMed : 33854239). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed : 30166453). With CUL4B, contributes to ribosome biogenesis (PubMed : 26711351).
See full target information Cullin-4A

Additional targets

CUL4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan
View all publications
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Product promise

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