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AB75812

Anti-Cullin 1/CUL-1 antibody [EPR3102Y]

5

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal Cullin 1/CUL-1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Cullin-1, CUL-1, CUL1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (AB75812)
  • ICC/IF

AbReview57480****

Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (AB75812)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cullin 1/CUL-1 with ab75812 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab75812 at 1/400 was carried in PBS buffer for 1 hour at 22°C. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.

This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (AB75812)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (AB75812)

ab75812 at 1/100 dilution staining Cullin 1/CUL-1 in HeLa cells by Immunofluorescence.

Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (AB75812)
  • WB

Unknown

Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (AB75812)

All lanes:

Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812) at 1/2000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

T47D cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 90 kDa

Observed band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3102Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed : 22017875, PubMed : 22017877, PubMed : 27565346). In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed : 38326650). The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1 (PubMed : 12609982, PubMed : 38326650). The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component (PubMed : 38326650). SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68 (PubMed : 25503564, PubMed : 25704143). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2. Neddylated CUL1-RBX1 ubiquitinates p53/TP53 recruited by Cul7-RING(FBXW8) complex (PubMed : 35982156). SCF(BTRC) directs 'Lys-48'-linked ubiquitination of UBR2 in the T-cell receptor signaling pathway (PubMed : 38225265).
See full target information CUL1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 80:2355-2367 PubMed32265224

2020

Targeted Inhibition of the E3 Ligase SCF Has Antitumor Activity in -Deficient Human and Mouse Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongling Zhao,Niloy J Iqbal,Vineeth Sukrithan,Cari Nicholas,Yingjiao Xue,Cindy Yu,Joseph Locker,Juntao Zou,Edward L Schwartz,Liang Zhu

PLoS pathogens 15:e1008086 PubMed31658303

2019

Nedd8 hydrolysis by UCH proteases in Plasmodium parasites.

Applications

Unspecified application

Species

Unspecified reactive species

Maryia Karpiyevich,Sophie Adjalley,Marco Mol,David B Ascher,Bethany Mason,Gerbrand J van der Heden van Noort,Heike Laman,Huib Ovaa,Marcus C S Lee,Katerina Artavanis-Tsakonas

Cell cycle (Georgetown, Tex.) 18:1135-1153 PubMed31057046

2019

Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dudley Chung,Jayme Salsman,Graham Dellaire

Journal of virology 88:3464-73 PubMed24403578

2014

Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH.

Applications

WB

Species

Unspecified reactive species

Markus Kainulainen,Matthias Habjan,Philipp Hubel,Laura Busch,Simone Lau,Jacques Colinge,Giulio Superti-Furga,Andreas Pichlmair,Friedemann Weber

Infection and immunity 82:72-82 PubMed24126522

2013

Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila.

Applications

Unspecified application

Species

Unspecified reactive species

William M Bruckert,Christopher T Price,Yousef Abu Kwaik

Molecular and cellular biology 33:1139-48 PubMed23297342

2013

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Chen,Honghui Ma,Hiroyuki Inuzuka,Jianbo Diao,Fei Lan,Yujiang Geno Shi,Wenyi Wei,Yang Shi
View all publications
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