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AB247585

Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Cullin 4A/CUL-4A antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.

View Alternative Names

Cullin-4A, CUL-4A, CUL4A

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

This data was developed using ab92554, the same antibody clone in a different buffer formulation.

ab92554 showing positive staining in Hepatocellular carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

This data was developed using ab92554, the same antibody clone in a different buffer formulation.

ab92554 showing positive staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

This data was developed using ab92554, the same antibody clone in a different buffer formulation.

ab92554 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

This data was developed using ab92554, the same antibody clone in a different buffer formulation.

ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection : DAB staining.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • IP

Lab

Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

This data was developed using ab92554, the same antibody clone in a different buffer formulation.

Cullin 4A/CUL-4A was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with 92554 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab92554 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab92554 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (<a href='/en-us/products/primary-antibodies/cullin-4a-cul-4a-antibody-epr3198-ab92554'>ab92554</a>)

Predicted band size: 88 kDa

false

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • WB

Supplier Data

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

Blocking buffer and concentration :  5% NFDM/TBST.  Diluting buffer and concentration :  5% NFDM/TBST. ab92554 recognizes  human Cul4A and Cul4B overexpression vectors. ab180602 was used as a loading control. This data was developed using ab92554, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (<a href='/en-us/products/primary-antibodies/cullin-4a-cul-4a-antibody-epr3198-ab92554'>ab92554</a>) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) transfected with blank vectors whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4B expression vectors whole cell lysate at 20 µg

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4A expression vectors whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 88 kDa,104 kDa

false

Exposure time: 1s

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)
  • WB

Unknown

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)

This data was developed using ab92554, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (<a href='/en-us/products/primary-antibodies/cullin-4a-cul-4a-antibody-epr3198-ab92554'>ab92554</a>) at 1/20000 dilution

Lane 1:

Hela cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3198

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 93% homology with Cul4B. Cross-reactivity with Cul4B has been observed using overexpression vectors but not on endogenous samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247585 is the carrier-free version of ab92554.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620, PubMed : 30166453, PubMed : 33854232, PubMed : 33854239). As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component (PubMed : 14578910, PubMed : 14739464, PubMed : 15448697, PubMed : 15548678, PubMed : 15811626, PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). DCX(DET1-COP1) directs ubiquitination of JUN (PubMed : 14739464). DCX(DDB2) directs ubiquitination of XPC (PubMed : 15811626). DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition (PubMed : 16678110, PubMed : 17041588, PubMed : 24209620). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53/TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed : 14578910, PubMed : 15448697, PubMed : 15548678). DCX(DTL) directs autoubiquitination of DTL (PubMed : 23478445). In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip (PubMed : 16537899). Is involved in ubiquitination of HOXA9 (PubMed : 14609952). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed : 26431207). A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed : 29779948). The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3) (PubMed : 33854232, PubMed : 33854239). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed : 30166453). With CUL4B, contributes to ribosome biogenesis (PubMed : 26711351).
See full target information Cullin-4A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:666-679 PubMed35170865

2022

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Huang,Ji-Xiong Chen,Yi Ren,Li-Chun Fan,Wei Xiang,Xiao-Jie He
View all publications
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