Rabbit Recombinant Monoclonal Cullin 4A/CUL-4A antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Transfected cell line - Human samples.
Images
![Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--western-blot-img106060.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67594.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)")
![Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunoprecipitation-img168092.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136183.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114875.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (AB247585)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Transfected cell line - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
Target data
Function
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins (PubMed:14578910, PubMed:14739464, PubMed:15448697, PubMed:15548678, PubMed:15811626, PubMed:16678110, PubMed:17041588, PubMed:24209620, PubMed:30166453, PubMed:33854232, PubMed:33854239). As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed:14578910, PubMed:14739464, PubMed:15448697, PubMed:15548678, PubMed:15811626, PubMed:16678110, PubMed:17041588, PubMed:24209620). The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (PubMed:14578910, PubMed:14739464, PubMed:15448697, PubMed:15548678, PubMed:15811626, PubMed:16678110, PubMed:17041588, PubMed:24209620). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component (PubMed:14578910, PubMed:14739464, PubMed:15448697, PubMed:15548678, PubMed:15811626, PubMed:16678110, PubMed:17041588, PubMed:24209620). DCX(DET1-COP1) directs ubiquitination of JUN (PubMed:14739464). DCX(DDB2) directs ubiquitination of XPC (PubMed:15811626). DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition (PubMed:16678110, PubMed:17041588, PubMed:24209620). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53/TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed:14578910, PubMed:15448697, PubMed:15548678). DCX(DTL) directs autoubiquitination of DTL (PubMed:23478445). In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip (PubMed:16537899). Is involved in ubiquitination of HOXA9 (PubMed:14609952). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948). The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3) (PubMed:33854232, PubMed:33854239). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed:30166453). With CUL4B, contributes to ribosome biogenesis (PubMed:26711351).
Alternative names
Cullin-4A, CUL-4A, CUL4A
Recommended products
Rabbit Recombinant Monoclonal Cullin 4A/CUL-4A antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3198
- Purification technique
- Affinity purification
- Specificity
The immunogen used for this product shares 93% homology with Cul4B. Cross-reactivity with Cul4B has been observed using overexpression vectors but not on endogenous samples.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247585 is the carrier-free version of Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--western-blot-img106060.jpg "Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554) at 1/20000 dilution
Lane 1: Hela cell lysate at 10 µg
Lane 2: A375 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 88 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67594.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunoprecipitation-img168092.jpg "Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.Cullin 4A/CUL-4A was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: abab92554 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554)
Predicted band size: 88 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136183.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 showing positive staining in Hepatocellular carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114875.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113185.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 showing positive staining in Ovarian carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.![Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--western-blot-img422188.jpg "Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554 recognizes human Cul4A and Cul4B overexpression vectors.
Anti-EBP1 antibody [EPR14569(B)] ab180602 was used as a loading control.
This data was developed using Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554, the same antibody clone in a different buffer formulation.All lanes: Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (Anti-Cullin 4A/CUL-4A antibody [EPR3198] ab92554) at 1/10000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) transfected with blank vectors whole cell lysate at 20 µg
Lane 2: HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4B expression vectors whole cell lysate at 20 µg
Lane 3: HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4A expression vectors whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa, 104 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113185.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
![Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585), expandable thumbnail](https://content.abcam.com/products/images/cullin-4a-cul-4a-antibody-epr3198-bsa-and-azide-free-ab247585--western-blot-img422188.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com