Rabbit Polyclonal Cullin 4B/CUL-4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CUL4B.
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Publications
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- iScience 26:1064682023Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael NaumannPubMed 37091236
- EMBO molecular medicine 15:e159242023Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Zhang,Gang Chen,Xing Feng,Huiwen Song,Lingbing Meng,Yao Fu,Jun Yang,Zhiwen Fan,Youxiang Ding,Zhijie Du,Jianchao Wang,Li Yang,Jun Zhang,Lixia Sun,Zhigang Liu,Zhiyong Zhang,Quanhai Li,Xiangshan FanPubMed 36947051
- Frontiers in pharmacology 12:7502332021The Traditional Chinese Medicine Compound Huangqin Qingre Chubi Capsule Inhibits the Pathogenesis of Rheumatoid Arthritis Through the CUL4B/Wnt Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Wang,Jun Chang,Guoliang Zhou,Chenglong Cheng,Youyi Xiong,Jinfeng Dou,Gen Cheng,Chenggui MiaoPubMed 34512369
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CUL4B. The exact immunogen used to generate this antibody is proprietary information. Database link Q13620
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14578910, PubMed:16322693, PubMed:16678110, PubMed:18593899, PubMed:22118460, PubMed:29779948, PubMed:30166453, PubMed:33854232, PubMed:33854239). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit (PubMed:14578910, PubMed:16678110, PubMed:18593899, PubMed:22118460, PubMed:29779948). CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed:14578910, PubMed:16678110, PubMed:18593899, PubMed:22118460). Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage (PubMed:14578910, PubMed:16678110, PubMed:18593899). Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication (PubMed:16678110). A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948). The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3) (PubMed:33854232, PubMed:33854239). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed:30166453). Required for ubiquitination of cyclin E (CCNE1 or CCNE2), and consequently, normal G1 cell cycle progression (PubMed:16322693, PubMed:19801544). Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism (PubMed:18235224). Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (PubMed:18235224). With CUL4A, contributes to ribosome biogenesis (PubMed:26711351).
Alternative names
KIAA0695, CUL4B, Cullin-4B, CUL-4B
Recommended products
Rabbit Polyclonal Cullin 4B/CUL-4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CUL4B.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CUL4B. The exact immunogen used to generate this antibody is proprietary information. Database link Q13620
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724)
5% SDS-PAGE
All lanes: Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
Lanes 1 and 4: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: C8D30 whole cell lysate at 30 µg
Lane 3: NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (ab227724)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cullin 4B/CUL-4B (green) using ab227724 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red - phalloidin cytoskeletal marker.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4B/CUL-4B antibody (ab227724)
Paraffin-embedded human esophagus carcinoma tissue stained for Cullin 4B/CUL-4B using ab227724 at 1/1000 dilution in immunohistochemical analysis.
Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724)
5% SDS-PAGE
All lanes: Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724)
5% SDS-PAGE
All lanes: Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724)
5% SDS-PAGE
All lanes: Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
All lanes: A-375 (human malignant melanoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
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