AB227724

Anti-Cullin 4B/CUL-4B antibody

Name: Anti-Cullin 4B/CUL-4B antibody (ab227724) | Abcam
Brand: Abcam Limited
SKU: AB227724
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Cullin 4B/CUL-4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CUL4B.

View Alternative Names

KIAA0695, CUL4B, Cullin-4B, CUL-4B

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (AB227724)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (AB227724)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cullin 4B/CUL-4B (green) using ab227724 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red - phalloidin cytoskeletal marker.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4B/CUL-4B antibody (AB227724)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Paraffin-embedded human esophagus carcinoma tissue stained for Cullin 4B/CUL-4B using ab227724 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

5% SDS-PAGE

All lanes:

Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 104 kDa

true

Supplier Data

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

5% SDS-PAGE

All lanes:

Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

All lanes:

A-375 (human malignant melanoma cell line) whole cell lysate at 30 µg

Predicted band size: 104 kDa

true

Supplier Data

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

5% SDS-PAGE

All lanes:

Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

Lanes 1 and 4:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Predicted band size: 104 kDa

true

Supplier Data

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

5% SDS-PAGE

All lanes:

Western blot - Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 104 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CUL4B. The exact immunogen used to generate this antibody is proprietary information.

Q13620

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14578910, PubMed : 16322693, PubMed : 16678110, PubMed : 18593899, PubMed : 22118460, PubMed : 29779948, PubMed : 30166453, PubMed : 33854232, PubMed : 33854239, PubMed : 25970626). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit (PubMed : 14578910, PubMed : 16678110, PubMed : 18593899, PubMed : 22118460, PubMed : 29779948). CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed : 14578910, PubMed : 16678110, PubMed : 18593899, PubMed : 22118460). Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage (PubMed : 14578910, PubMed : 16678110, PubMed : 18593899). Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication (PubMed : 16678110). A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed : 29779948). The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3) (PubMed : 33854232, PubMed : 33854239). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed : 30166453). Required for ubiquitination of cyclin E (CCNE1 or CCNE2), and consequently, normal G1 cell cycle progression (PubMed : 16322693, PubMed : 19801544). Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism (PubMed : 18235224). Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (PubMed : 18235224). With CUL4A, contributes to ribosome biogenesis (PubMed : 26711351).

See full target information CUL4B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

Science signaling 17:eadg7867 PubMed38593156

2024

Miz1 represses type I interferon production and limits viral clearance during influenza A virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjiao Wu,Vinothini Arunagiri,Hanh Chi Do-Umehara,Cong Chen,Shuyin Gu,Indrani Biswas,Karen M Ridge,G R Scott Budinger,Shuwen Liu,Jing Liu

iScience 26:106468 PubMed37091236

2023

Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.

Applications

Unspecified application

Species

Unspecified reactive species

Dawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael Naumann

EMBO molecular medicine 15:e15924 PubMed36947051

2023

Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Gang Chen,Xing Feng,Huiwen Song,Lingbing Meng,Yao Fu,Jun Yang,Zhiwen Fan,Youxiang Ding,Zhijie Du,Jianchao Wang,Li Yang,Jun Zhang,Lixia Sun,Zhigang Liu,Zhiyong Zhang,Quanhai Li,Xiangshan Fan

Bioscience reports 42: PubMed35880551

2022

Inhibition of NEDD8 NEDDylation induced apoptosis in acute myeloid leukemia cells via p53 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Chen,Ling Sun

Frontiers in pharmacology 12:750233 PubMed34512369

2021

The Traditional Chinese Medicine Compound Huangqin Qingre Chubi Capsule Inhibits the Pathogenesis of Rheumatoid Arthritis Through the CUL4B/Wnt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wang,Jun Chang,Guoliang Zhou,Chenglong Cheng,Youyi Xiong,Jinfeng Dou,Gen Cheng,Chenggui Miao

Cancer cell international 20:572 PubMed33292255

2020

Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Du,Lei Wang,Yu Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cullin 4B/CUL-4B antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Western blot - Anti-Cullin 4B/CUL-4B antibody (AB227724)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise