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Rabbit Recombinant Monoclonal CUTC antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (AB248644), expandable thumbnail
  • Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (AB248644), expandable thumbnail
  • OI-RD Scanning - Anti-CUTC antibody [EPR7929] - BSA and Azide free (AB248644), expandable thumbnail
  • Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (AB248644), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

May play a role in copper homeostasis. Can bind one Cu(1+) per subunit.

Alternative names

CGI-32, CUTC, Copper homeostasis protein cutC homolog

Recommended products

Rabbit Recombinant Monoclonal CUTC antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7929
Purification technique
Affinity purification
Dissociation constant
2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248644 is the carrier-free version of Anti-CUTC antibody [EPR7929] ab133762.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CUTC also known as copper homeostasis protein CUTC plays a role in copper transport and homeostasis. It has a molecular mass of approximately 30 kDa. CUTC expression occurs mainly in the liver kidney and intestines suggesting its importance in managing systemic copper levels. CUTC contains several copper-binding motifs that help it in coordinating and potentially transporting copper ions across cellular compartments.

Biological function summary

Copper homeostasis protein CUTC functions in the regulation of intracellular copper levels and may interact with copper chaperones. The protein does not work in isolation but instead might form complexes with other copper-binding proteins to facilitate efficient copper regulation. Proper function of CUTC is necessary for various copper-dependent enzymatic activities highlighting its role in maintaining cellular copper sufficiency.

Pathways

CUTC is engaged in the copper homeostasis pathway that ensures adequate copper distribution and detoxification. This protein is associated with other copper-related proteins like ATP7A/B which transport copper across cellular membranes. Through these interactions CUTC assists in protecting cells from copper toxicity and supports essential biological functions requiring copper.

Associated diseases and disorders

CUTC relates to Wilson's disease and Menkes disease which are disorders of copper metabolism. CUTC interacts with proteins such as ATP7B in Wilson's disease and ATP7A in Menkes disease. Understanding CUTC's function and interactions is key for developing therapeutic strategies aimed at alleviating symptoms associated with impaired copper homeostasis in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644), expandable thumbnail

    Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644)

    This data was developed using Anti-CUTC antibody [EPR7929] ab133762, the same antibody clone in a different buffer formulation.

    Lanes 1-5: Merged signal (red and green). Green - Anti-CUTC antibody [EPR7929] ab133762 observed at 29 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    Anti-CUTC antibody [EPR7929] ab133762 Anti-CUTC antibody [EPR7929] was shown to specifically react with CUTC in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CUTC knockout HeLa cell line ab265860 (knockout cell lysate Human CUTC knockout HeLa cell lysate ab258388) was used. Wild-type and CUTC knockout samples were subjected to SDS-PAGE. Anti-CUTC antibody [EPR7929] ab133762 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CUTC antibody [EPR7929] (Anti-CUTC antibody [EPR7929] ab133762) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CUTC knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human CUTC knockout HeLa cell line (Human CUTC knockout HeLa cell line ab265860)

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: SH-SY5Y cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644), expandable thumbnail

    Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644)

    This data was developed using Anti-CUTC antibody [EPR7929] ab133762, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CUTC antibody [EPR7929] (Anti-CUTC antibody [EPR7929] ab133762) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • OI-RD Scanning - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644), expandable thumbnail

    OI-RD Scanning - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644), expandable thumbnail

    Western blot - Anti-CUTC antibody [EPR7929] - BSA and Azide free (ab248644)

    This data was developed using Anti-CUTC antibody [EPR7929] ab133762, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-CUTC antibody [EPR7929] (Anti-CUTC antibody [EPR7929] ab133762) at 1/2000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Lane 6: Rat heart tissue lysate at 20 µg

    Lane 7: Rat kidney tissue lysate at 20 µg

    Lane 8: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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