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AB188345

Anti-CWC15 antibody

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(2 Publications )

Rabbit Polyclonal CWC15 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CWC15 aa 1-100.

View Alternative Names

C11orf5, AD-002, HSPC148, CWC15, Spliceosome-associated protein CWC15 homolog

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CWC15 antibody (AB188345)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CWC15 antibody (AB188345)

Paraffin embedded human thyroid gland tissue stained for CWC15 using ab188345 at 1/1000 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CWC15 antibody (AB188345)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CWC15 antibody (AB188345)

U-2 OS cells stained for CWC15 (green) using ab188345 at 2 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CWC15 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9P013

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CWC15 also known as CWC15 spliceosome-associated protein homolog plays a role in RNA splicing. It is a protein with a mass of approximately 16 kDa. CWC15 is expressed in various human tissues but it shows higher expression in hematopoietic cells. Its mechanical function involves facilitating the splicing of pre-mRNA by associating with the spliceosome a large ribonucleoprotein complex.
Biological function summary

CWC15 contributes to RNA processing within cells. It forms part of the spliceosome complex which is critical for the modification of pre-mRNA to mature mRNA. This modification process is necessary for proper gene expression and cellular function. The influence of CWC15 in this complex underlines its importance in regulating the correct removal of introns and the joining of exons ensuring that mature mRNA accurately reflects genetic coding.

Pathways

CWC15 has connections to the mRNA splicing pathway and RNA transport. It interacts with other spliceosomal proteins like PRPF8 and SNRNP200 facilitating efficient splicing activity. These interactions ensure the proper flow of genetic information from DNA to functional proteins impacting the gene expression pathway that maintains cellular homeostasis.

CWC15 has associations with myelodysplastic syndromes and acute myeloid leukemia. These diseases relate to its role in RNA splicing which if altered can lead to abnormalities in hematopoietic cells. Its interaction with PRPF8 a protein implicated in similar conditions suggests a critical contribution of CWC15 to maintaining normal cellular function and preventing malignancies in hematopoietic tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 28076346, PubMed : 28502770). Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
See full target information CWC15
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:315 PubMed39747150

2025

CDC40 suppression induces CDCA5 splicing defects and anti-proliferative effects in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Die Hu,Brigitte L Thériault,Vida Talebian,Laurent Hoffer,Julie Owen,Justin Lim,Benjamin J Blencowe,Evelyne Lima-Fernandes,Punit Saraon,Richard Marcellus,Rima Al-Awar

Frontiers in oncology 10:584024 PubMed33634013

2021

The Cancer/Testis Antigen Gene Is Rarely Expressed in Malignancies but Can Be Epigenetically Activated Using DNA Methyltransferase and Histone Deacetylase Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Mie K Jakobsen,Sofie Traynor,Mette Stæhr,Pascal G Duijf,Aaraby Y Nielsen,Mikkel G Terp,Christina B Pedersen,Per Guldberg,Henrik J Ditzel,Morten F Gjerstorff
View all publications
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Product promise

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