Rabbit Polyclonal CX3CL1 antibody. Suitable for IHC-P, WB, IHC-FoFr, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 68 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CX3CL1.
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- Molecular medicine (Cambridge, Mass.) 29:1402023Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiming Gong,Tengfang Lai,Liudan Liang,Yan Jiang,Fahui LiuPubMed 37875838
- Scientific reports 12:149802022Polyphenolic grape stalk and coffee extracts attenuate spinal cord injury-induced neuropathic pain development in ICR-CD1 female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Bagó-Mas,Andrea Korimová,Meritxell Deulofeu,Enrique Verdú,Núria Fiol,Viktorie Svobodová,Petr Dubový,Pere Boadas-VaelloPubMed 36056079
- Microbiology spectrum 10:e01091222022SARS-CoV-2 Infection of Microglia Elicits Proinflammatory Activation and Apoptotic Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGi Uk Jeong,Jaemyun Lyu,Kyun-Do Kim,Young Cheul Chung,Gun Young Yoon,Sumin Lee,Insu Hwang,Won-Ho Shin,Junsu Ko,June-Yong Lee,Young-Chan KwonPubMed 35510852
- International journal of molecular sciences 23:2022Berberine Suppresses Leukocyte Adherence by Downregulating CX3CL1 Expression and Shedding and ADAM10 in Lipopolysaccharide-Stimulated Vascular Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Hong Wu,Chen-Ying Wei,Wei-Chin Hong,Jong-Hwei Su PangPubMed 35563195
- Journal of healthcare engineering 2022:25804192022Expression of CX3CL1 and CCL28 in Spinal Metastases of Lung Adenocarcinoma and Their Correlation with Clinical Features and Prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuguang Li,Shuai Liu,Jianjun Xun,Feifei ZhaoPubMed 35494513
- Neoplasma 69:670-6792022CX3CL1 in the red bone marrow promotes renal cell carcinoma to metastasize to the spine by involving the Src-related pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn-Nan Hu,Fan-Cheng Chen,Ke-Tao Wang,Zhen-Qing Wang,Yun Liang,Jian DongPubMed 35330998
- Journal of clinical medicine 10:2021Is Saffron Able to Prevent the Dysregulation of Retinal Cytokines Induced by Ocular Hypertension in Mice?Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé A Fernández-Albarral,Miguel A Martínez-López,Eva M Marco,Rosa de Hoz,Beatriz Martín-Sánchez,Diego San Felipe,Elena Salobrar-García,Inés López-Cuenca,María D Pinazo-Durán,Juan J Salazar,José M Ramírez,Meritxell López-Gallardo,Ana I RamírezPubMed 34768320
- Journal of inflammation research 14:2061-20782021Upregulation of Neuronal Cylindromatosis Expression is Essential for Electroacupuncture-Mediated Alleviation of Neuroinflammatory Injury by Regulating Microglial Polarization in Rats Subjected to Focal Cerebral Ischemia/Reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing Lin,Jian Zhan,Jin Jiang,Yikun RenPubMed 34045881
- Journal of ethnopharmacology 275:1141752021Jinfukang inhibits lung cancer metastasis by upregulating CX3CL1 to recruit NK cells to kill CTCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZu-Jun Que,Jia-Liang Yao,Zhi-Yi Zhou,Pan Yu,Bin Luo,He-Gen Li,Jia-Xiang Liu,Hong-Xi Xu,Jian-Hui TianPubMed 33933571
- The European journal of neuroscience :2021Significant higher-level C-C motif chemokine ligand 2/3 and chemotactic power in cerebral white matter than grey matter in rat and human.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingdong Zhang,Xinrui Gong,Huangui XiongPubMed 33725384
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.5
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CX3CL1. The exact immunogen used to generate this antibody is proprietary information. Database link P78423
Reactivity data
Select an application| IHC-P | WB | IHC-FoFr | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested | Expected |
| Mouse | Predicted | Expected | Expected | Predicted | Expected |
| Rat | Predicted | Expected | Expected | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1 (PubMed:12055230, PubMed:21829356, PubMed:23125415, PubMed:9782118, PubMed:9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:12055230, PubMed:9024663, PubMed:9177350, PubMed:9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed:23125415, PubMed:24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed:23125415, PubMed:24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed:27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
Alternative names
FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1
Recommended products
Rabbit Polyclonal CX3CL1 antibody. Suitable for IHC-P, WB, IHC-FoFr, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 68 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CX3CL1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.5
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CX3CL1. The exact immunogen used to generate this antibody is proprietary information. Database link P78423
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.
- Biological function summary
CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.
- Pathways
CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.
- Associated diseases and disorders
CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
ab25088 (4µg/ml) staining CX3CL1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic region of the cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)
ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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