AB25088

Anti-CX3CL1 antibody

Name: Anti-CX3CL1 antibody (ab25088) | Abcam
Brand: Abcam Limited
SKU: AB25088
Price: 450 USD
Availability: InStock
Rating: 3 (6 reviews)

(6 Reviews)

(85 Publications)

Anti-CX3CL1 antibody (ab25088) is a rabbit polyclonal antibody detecting CX3CL1 in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 60 publications
- Trusted since 2006

View Alternative Names

FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (AB25088)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (AB25088)

ab25088 (4µg/ml) staining CX3CL1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic region of the cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (AB25088)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (AB25088)

ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-FoFr, IHC-P, ICC/IF, WB, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human CX3CL1. The exact immunogen used to generate this antibody is proprietary information.

P78423

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CX3CL1 antibody (ab25088) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-CX3CL1 (ab25088) was first used in a scientific publication in 2006 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists
Anti-CX3CL1 (ab25088) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 6.5 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.

Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1 (PubMed : 12055230, PubMed : 21829356, PubMed : 23125415, PubMed : 9782118, PubMed : 9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed : 12055230, PubMed : 9024663, PubMed : 9177350, PubMed : 9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed : 9024663, PubMed : 9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed : 23125415, PubMed : 24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed : 23125415, PubMed : 24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed : 23125415, PubMed : 24789099).. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed : 27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).

See full target information CX3CL1

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Publications (85)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:924 PubMed40826460

2025

AAV-mediated STC-1 expression mitigates neuroinflammation and preserves visual function in degenerative retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yange Wang,Siyu Li,Chenxu Zhang,Miao Li,Kexin Wang,Gang Wang,Ye Tao,Zongming Song

Stem cells translational medicine 14: PubMed40110808

2025

Mitigating murine acute and chronic Coxsackievirus B3-induced myocarditis with human right atrial appendage-derived stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad El-Shafeey,Kathleen Pappritz,Isabel Voss,Kapka Miteva,Alessio Alogna,Martina Seifert,Henry Fechner,Jens Kurreck,Karin Klingel,Marion Haag,Michael Sittinger,Carsten Tschöpe,Sophie Van Linthout

Journal of neuroinflammation 22:77 PubMed40075472

2025

Centripetal migration and prolonged retention of microglia promotes spinal cord injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jianan Ye,Fangli Shan,Xinzhong Xu,Chao Liang,Ningyuan Zhang,Hao Hu,Jianjian Li,Fangru Ouyang,Jingwen Wang,Yuanzhe Zhao,Zhida Ma,Congpeng Meng,Ziyu Li,Shuisheng Yu,Juehua Jing,Meige Zheng

Stem cell research & therapy 16:124 PubMed40055828

2025

Effects and mechanisms of breastmilk stem cells in the treatment of white matter injury in newborn rats.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhang,Haoran Wang,Yang He,Wenxing Li,Hongju Chen,Xinyu Zhang,Qiang Chen,Chao Yang,Maowen Luo,Bo Zhang,Jun Tang,Dezhi Mu

Stem cell research & therapy 15:356 PubMed39385216

2024

The therapeutic use of clonal neural stem cells in experimental Parkinson´s disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Nelke,Silvia García-López,Javier R Caso,Marta P Pereira

Cell communication and signaling : CCS 22:457 PubMed39327578

2024

Fractalkine/CX3CR1 axis is critical for neuroprotection induced by hypoxic postconditioning against cerebral ischemic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lixuan Zhan,Meiqian Qiu,Jianhua Zheng,Meijing Lai,Kunqin Lin,Jiahua Dai,Weiwen Sun,En Xu

Inflammation and regeneration 44:30 PubMed38844990

2024

CX3CL1-CX3CR1 axis protects retinal ganglion cells by inhibiting microglia activation in a distal optic nerve trauma model.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Yu,Bingqiao Shen,Ruiqi Han,Yang Zhang,Shushu Xu,Yumeng Zhang,Yanzhi Guo,Ping Huang,Shouyue Huang,Yisheng Zhong

JCI insight 9: PubMed38775151

2024

Double-faced CX3CL1 enhances lymphangiogenesis-dependent metastasis in an aggressive subclone of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Htoo Shwe Eain,Hotaka Kawai,Masaaki Nakayama,May Wathone Oo,Toshiaki Ohara,Yoko Fukuhara,Kiyofumi Takabatake,Quisheng Shan,Yamin Soe,Kisho Ono,Keisuke Nakano,Nobuyoshi Mizukawa,Seiji Iida,Hitoshi Nagatsuka

Heliyon 10:e29100 PubMed38601629

2024

CX3CL1 and its receptor CX3CR1 interact with RhoA signaling to induce paclitaxel resistance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyang Liu,Zhonghui Yu,Yun Li,Junzi Huang

Investigative ophthalmology & visual science 65:29 PubMed38231527

2024

CX3CL1/CX3CR1 Signaling Mediated Neuroglia Activation Is Implicated in the Retinal Degeneration: A Potential Therapeutic Target to Prevent Photoreceptor Death.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Min Huang,Na Zhao,Xiao-Na Hao,Si-Yu Li,Dong Wei,Ning Pu,Guang-Hua Peng,Ye Tao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CX3CL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (AB25088)

Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (AB25088)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (85)

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