Mouse Monoclonal CX3CL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CX3CL1.
Images
![Western blot - Anti-CX3CL1 antibody [MM0207-8J23] (AB89229), expandable thumbnail](https://content.abcam.com/products/images/cx3cl1-antibody-mm0207-8j23-ab89229--western-blot-img179466.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CX3CL1 antibody [MM0207-8J23] (AB89229)")
Publications
Filter by
- Neoplasma 69:670-6792022CX3CL1 in the red bone marrow promotes renal cell carcinoma to metastasize to the spine by involving the Src-related pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn-Nan Hu,Fan-Cheng Chen,Ke-Tao Wang,Zhen-Qing Wang,Yun Liang,Jian DongPubMed 35330998
- International journal of oncology 57:249-2632020ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Sun et. al.PubMed 32319605
- Clinical cancer research : an official journal of the American Association for Cancer Research 20:3094-1062014GM-CSF Production by Tumor Cells Is Associated with Improved Survival in Colorectal Cancer.Applications:Unspecified applicationReactive species:HumanChristian A Nebiker,Junyi Han,Serenella Eppenberger-Castori,Giandomenica Iezzi,Christian Hirt,Francesca Amicarella,Eleonora Cremonesi,Xaver Huber,Elisabetta Padovan,Basilio Angrisani,Raoul A Droeser,Raffaele Rosso,Martin Bolli,Daniel Oertli,Urs von Holzen,Michel Adamina,Manuele G Muraro,Chantal Mengus,Paul Zajac,Giuseppe Sconocchia,Markus Zuber,Luigi Tornillo,Luigi Terracciano,Giulio C SpagnoliPubMed 24737547
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CX3CL1. Database link P78423
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1 (PubMed:12055230, PubMed:21829356, PubMed:23125415, PubMed:9782118, PubMed:9931005). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:12055230, PubMed:9024663, PubMed:9177350, PubMed:9782118). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed:23125415, PubMed:24789099). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed:23125415, PubMed:24789099). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes (PubMed:27653778). The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
Alternative names
FKN, NTT, SCYD1, A-152E5.2, CX3CL1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1
Recommended products
Mouse Monoclonal CX3CL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CX3CL1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CX3CL1. Database link P78423
- Clone number
- MM0207-8J23
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.
- Biological function summary
CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.
- Pathways
CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.
- Associated diseases and disorders
CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-CX3CL1 antibody [MM0207-8J23] (ab89229), expandable thumbnail](https://content.abcam.com/products/images/cx3cl1-antibody-mm0207-8j23-ab89229--western-blot-img179466.jpg "Western blot - Anti-CX3CL1 antibody [MM0207-8J23] (ab89229)")
Western blot - Anti-CX3CL1 antibody [MM0207-8J23] (ab89229)
All lanes: Western blot - Anti-CX3CL1 antibody [MM0207-8J23] (ab89229) at 1/500 dilution
All lanes: Human placenta tissue lysate
Predicted band size: 42 kDa
Observed band size: 35 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com