Anti-Cx40/GJA5 antibody [EPR28370-62]

10 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Cx40/GJA5 with ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse pancreas.The section was incubated with ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunoprecipitation - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Cx40/GJA5 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate with ab313644 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313644 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: Mouse lung tissue lysate
  Lane 2: ab313644 IP in Mouse lung tissue lysate
  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313644 in mouse lung tissue lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 24 seconds

  All lanes: Immunoprecipitation - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644) at 1/30 dilution

  All lanes: Mouse lung tissue lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

  Exposure time: 24s

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  Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST#Negative control: colon, stomach
  The molecular weight observed is consistent with what has been described in the literature (PMID: 29285063, 19726871).
  Samples are non-boiled as boiling may cause protein aggregation.
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: Lane1-3: 37 seconds; Lane 4: 10 seconds

  All lanes: Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644) at 1/1000 dilution

  Lane 1: Mouse heart tissue lysate at 20 µg

  Lane 2: Mouse colon tissue lysate at 20 µg

  Lane 3: Mouse stomach tissue lysate at 20 µg

  Lane 4: Mouse lung tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 40, 36 kDa

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  Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  The molecular weight observed is consistent with what has been described in the literature (PMID: 29285063, 19726871).
  Samples are non-boiled as boiling may cause protein aggregation.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (Anti-Integrin alpha V antibody [EPR16800] ab179475) control staining at 1/5000 dilution
  Exposure time: 37 seconds

  All lanes: Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644) at 1/1000 dilution

  Lane 1: Mouse lung non-membrane fraction at 20 µg

  Lane 2: Mouse lung membrane fraction at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 40, 36 kDa

  Exposure time: 37s

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  Immunohistochemistry (Frozen sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas (fresh) tissue labeling Cx40/GJA5 with ab313644 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse pancreas. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313644 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh) tissue labeling Cx40/GJA5 with ab313644 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313644 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling Cx40/GJA5 with ab313644 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on endothelium of mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313644 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cx40/GJA5 with ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on endothelium of mouse lung (PMID: 17483738).The section was incubated with ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Cx40/GJA5 with ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney juxtaglomerulus (PMID: 29427253).The section was incubated with ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] (ab313644)

  Immunohistochemical analysis of paraffin-embedded Mouse atrium cardiac tissue labeling Cx40/GJA5 with ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse atrium cardiac muscle (PMID: 30564139, 17122196).The section was incubated with ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins