Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)

Rabbit Recombinant Monoclonal Cx40/GJA5 antibody. Carrier free. Suitable for WB, IP, IHC-P, IHC-Fr and reacts with Mouse samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (AB313645), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	IHC-P	IHC-Fr	Flow Cyt (Intra)	ICC/IF
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Gja5

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Alternative names

Cxn-40, Gja5, Gap junction alpha-5 protein, Connexin-40, Cx40

Recommended products

Rabbit Recombinant Monoclonal Cx40/GJA5 antibody. Carrier free. Suitable for WB, IP, IHC-P, IHC-Fr and reacts with Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28370-62
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse pancreas.The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Cx40/GJA5 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 IP in Mouse lung tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 in mouse lung tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
All lanes: Immunoprecipitation - Anti-Cx40/GJA5 antibody [EPR28370-62] (Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644) at 1/30 dilution
All lanes: Mouse lung tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Exposure time: 24s
Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST#Negative control: colon, stomach
The molecular weight observed is consistent with what has been described in the literature (PMID: 29285063, 19726871).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: Lane1-3: 37 seconds; Lane 4: 10 seconds
All lanes: Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 20 µg
Lane 2: Mouse colon tissue lysate at 20 µg
Lane 3: Mouse stomach tissue lysate at 20 µg
Lane 4: Mouse lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40, 36 kDa
Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 29285063, 19726871).
Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti-Integrin alpha V (Anti-Integrin alpha V antibody [EPR16800] ab179475) control staining at 1/5000 dilution
Exposure time: 37 seconds
All lanes: Western blot - Anti-Cx40/GJA5 antibody [EPR28370-62] (Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644) at 1/1000 dilution
Lane 1: Mouse lung non-membrane fraction at 20 µg
Lane 2: Mouse lung membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40, 36 kDa
Exposure time: 37s
Immunohistochemistry (Frozen sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas (fresh) tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse pancreas. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh) tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on endothelium of mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on endothelium of mouse lung (PMID: 17483738).The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney juxtaglomerulus (PMID: 29427253).The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx40/GJA5 antibody [EPR28370-62] - BSA and Azide free (ab313645)
This data was developed using Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse atrium cardiac tissue labeling Cx40/GJA5 with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse atrium cardiac muscle (PMID: 30564139, 17122196).The section was incubated with Anti-Cx40/GJA5 antibody [EPR28370-62] ab313644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins