AB222885

Anti-Cx50 antibody

Name: Anti-Cx50 antibody (ab222885) | Abcam
Brand: Abcam Limited
SKU: AB222885
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal Cx50 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Gap junction alpha-8 protein aa 250 to C-terminus.

View Alternative Names

Gap junction alpha-8 protein, Connexin-50, Lens fiber protein MP70, Cx50, GJA8

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx50 antibody (AB222885)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx50 antibody (AB222885)

Paraffin-embedded human breast cancer tissue stained for Cx50 using ab222885 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Cx50 antibody (AB222885)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cx50 antibody (AB222885)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Cx50 (green) using ab222885 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx50 antibody (AB222885)

Paraffin-embedded human liver cancer tissue stained for Cx50 using ab222885 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Cx50 antibody (AB222885)

All lanes:

Western blot - Anti-Cx50 antibody (ab222885) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 3:

Rat brain lysate

Lane 4:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Gap junction alpha-8 protein aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48165

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Structural component of eye lens gap junctions (PubMed : 18006672, PubMed : 19756179). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed : 18006672, PubMed : 19756179).

See full target information Gap junction alpha-8 protein

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 64:15 PubMed38095908

2023

JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Sun,Jiani Li,Jingyu Ma,Yuxing Zheng,Rong Ju,Xuri Li,Xiangrong Ren,Lijuan Huang,Rongyuan Chen,Xuhua Tan,Lixia Luo

BMC ophthalmology 20:461 PubMed33218330

2020

Identification and functional analysis of a novel missense mutation in GJA8, p.Ala69Thr.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Li,Chenjia Xu,Dandan Huang,Ruru Guo,Jian Ji,Wei Liu

Laboratory investigation; a journal of technical m 99:1714-1727 PubMed30683901

2019

Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Yu,Ziyan Yu,Danhong Wu,Jiao Zhang,Ying Zhu,Yang Zhang,Hong Ning,Mingwu Wang,Jinsong Zhang,Jiangyue Zhao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cx50 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx50 antibody (AB222885)

Immunocytochemistry/ Immunofluorescence - Anti-Cx50 antibody (AB222885)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx50 antibody (AB222885)

Western blot - Anti-Cx50 antibody (AB222885)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise