AB86436

Anti-CXCL1/GRO alpha antibody

(1 Review)

(56 Publications)

Rabbit Polyclonal CXCL1/GRO alpha antibody. Suitable for WB and reacts with Recombinant full length protein - Rat, Mouse, Human samples. Cited in 56 publications. Immunogen corresponding to Synthetic Peptide within Mouse Cxcl1.

View Alternative Names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

2 Images

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Western blot - Anti-CXCL1/GRO alpha antibody (AB86436)

All lanes:

Western blot - Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/mL

Lane 1:

Jurkat cell lysate

Lane 2:

3T3 cell lysate

Predicted band size: 11 kDa

Observed band size: 9 kDa

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Western blot - Anti-CXCL1/GRO alpha antibody (AB86436)

All lanes:

Western blot - Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/mL

Lane 1:

Western blot - Recombinant rat CXCL1/GRO alpha protein (<a href='/en-us/products/proteins-peptides/recombinant-rat-cxcl1-gro-alpha-protein-ab9773'>ab9773</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant rat CXCL1/GRO alpha protein (<a href='/en-us/products/proteins-peptides/recombinant-rat-cxcl1-gro-alpha-protein-ab9773'>ab9773</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Mouse Cxcl1. The exact immunogen used to generate this antibody is proprietary information.

P12850

Specificity

The immunogen for ab86436 has >90% homology to mouse GRO beta

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 5212 KC Antibody.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate) Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.

Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

See full target information CXCL1

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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1470625 PubMed40061942

2025

Role of RGS17 in cisplatin-induced cochlear inflammation and ototoxicity via caspase-3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Raheem F H Al Aameri,Entkhab M A Alanisi,Dheyaa Al Sallami,Ian Alberts,Shelley Tischkau,Leonard P Rybak,Vickram Ramkumar

Frontiers in immunology 15:1513196 PubMed39902039

2025

Inflammatory biomarkers and therapeutic potential of milk exosome-mediated CCL7 siRNA in murine intestinal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

WenDong Chen,WenPing Xu,Li Ma,Chun Bi,MeiXu Yang,Wei Yang

Journal of ovarian research 18:3 PubMed39773697

2025

Association between cytokines and progression-free survival in ovarian cancer following CRS/HIPEC treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yu Chen,Ting-Yao Wang,Jing-Lan Liu,Yu-Che Ou,Li-Wen Lee,Chien-Hui Hung,Chuan-Pin Lee,Jrhau Lung

Nature communications 15:6718 PubMed39112467

2024

Skin hepcidin initiates psoriasiform skin inflammation via Fe-driven hyperproliferation and neutrophil recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Elise Abboud,Doha Chrayteh,Nadia Boussetta,Héloise Dalle,Mariangela Malerba,Ting-Di Wu,Morgane Le Gall,Olivier Reelfs,Charareh Pourzand,Mark Mellett,Florence Assan,Hervé Bachelez,Joël Poupon,Selim Aractingi,Sophie Vaulont,Pierre Sohier,Bénédicte Oules,Zoubida Karim,Carole Peyssonnaux

International journal of molecular sciences 25: PubMed39062946

2024

The Variation in the Traits Ameliorated by Inhibitors of JAK1/2, TGF-β, P-Selectin, and CXCR1/CXCR2 in the Model Suggests That Myelofibrosis Should Be Treated by These Drugs in Combination.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Gobbo,Fabrizio Martelli,Antonio Di Virgilio,Elena Demaria,Giuseppe Sarli,Anna Rita Migliaccio

Cell death & disease 15:447 PubMed38918360

2024

IGFBP2/ITGA5 promotes gefitinib resistance via activating STAT3/CXCL1 axis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hengxiao Lu,Jiangshan Ai,Yingying Zheng,Wolong Zhou,Liming Zhang,Jiebo Zhu,Heng Zhang,Shaoqiang Wang

Aging cell 23:e14193 PubMed38724466

2024

Non-pituitary growth hormone enables colon cell senescence evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Chesnokova,Svetlana Zonis,Tugce Apaydin,Robert Barrett,Shlomo Melmed

Discover oncology 15:88 PubMed38536591

2024

Circ_0020460 drives tumorigenesis in cervical cancer through miR-485-3p sponging.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yan,Chunyan Hu,Yali Cheng,Lingzhi Zheng,Baojin Zeng,Sujun Zhao,Chen Liu

Communications biology 6:516 PubMed37179352

2023

Vascular cell-adhesion molecule 1 (VCAM-1) regulates JunB-mediated IL-8/CXCL1 expression and pathological neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Geetika Kaur,Deepti Sharma,Shivantika Bisen,Chandra Sekhar Mukhopadhyay,Katherine Gurdziel,Nikhlesh K Singh

Fundamental research 5:1804-1821 PubMed40777809

2023

Stem cell-derived small extracellular vesicles containing miR-27b-3p attenuated osteoarthritis through inhibition of leukaemia inhibitory factor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoguang Zhang,Wei Chen,Shenghui Lan,Yuxiang Hu,Hongxin Pei,Zhili He,Zhipeng Dai,Yulong Wei,Zhenxing Wang,Qinyu Ma,Fenfei Zhao,Juan Wang,Zengwu Shao,Yong Liu,Shuhua Yang,Hongtao Tian,Wei Tong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL1/GRO alpha antibody

Western blot - Anti-CXCL1/GRO alpha antibody (AB86436)

Western blot - Anti-CXCL1/GRO alpha antibody (AB86436)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (56)

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