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Rabbit Recombinant Monoclonal CXCL1/GRO alpha antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications.

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Images

Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (AB206411), expandable thumbnail
  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (AB206411), expandable thumbnail
  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (AB206411), expandable thumbnail
  • Immunoprecipitation - Anti-CXCL1/GRO alpha antibody [EPR19892] (AB206411), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Recombinant fragment - Human
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Alternative names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

Recommended products

Rabbit Recombinant Monoclonal CXCL1/GRO alpha antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19892
Purification technique
Affinity purification Protein A
Specificity

This antibody cross-reacts with recombinant human CXCL2 and CXCL3 protein.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.

Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

Associated diseases and disorders

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411), expandable thumbnail

    Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

    False colour image of Western blot: Anti-CXCL1/GRO alpha antibody [EPR19892] staining at 1/100 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab206411 was shown to bind specifically to CXCL1/GRO alpha. A band was observed at 8 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in CXCL1 knockout cell line Human CXCL1 (GRO alpha) knockout HeLa cell line ab261774 (knockout cell lysate Human CXCL1 (GRO alpha) knockout HeLa cell lysate ab257079). To generate this image, wild-type and CXCL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.

    All lanes: Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution

    Lane 1: Wild-type HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate at 20 µg

    Lane 2: CXCL1 knockout HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate at 20 µg

    Lane 2: Western blot - Human CXCL1 (GRO alpha) knockout HeLa cell line (Human CXCL1 (GRO alpha) knockout HeLa cell line ab261774)

    Performed under reducing conditions.

    Predicted band size: 11 kDa

    Observed band size: 8 kDa

  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411), expandable thumbnail

    Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human GRO beta fragment recombinant protein contain aa35-107 with a GST-Tag. Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag®. These two protein were made in house.

    All lanes: Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/1000 dilution

    Lane 1: Human CXCL1/GRO alpha active protein at 0.01 µg

    Lane 2: Human GRO beta fragment recombinant protein at 0.01 µg

    Lane 3: Human GRO gamma fragment recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 10 kDa, 36 kDa

    Exposure time: 3min

  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411), expandable thumbnail

    Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution

    All lanes: Human fetal lung lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 11 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411), expandable thumbnail

    Immunoprecipitation - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

    11 kDa CXCL1/GRO alpha was immunoprecipitated from 0.35mg of HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab206411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1 (Input): HUVEC treated with 0.5 μg/ml LPS for 4 hours, then added 300 ng/ml BFA for another 20 hours whole cell lysate 10 μg.

    Lane 2 (+): ab206411-IP in HUVEC treated with 0.5 μg/ml LPS for 4 hours, then added 300 ng/ml BFA for another 20 hours whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab206411 in HUVEC treated as above whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

    All lanes: Immunoprecipitation - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

    Lane 1: HUVEC (human umbilical vein endothelial cell) treated with 0.5 μg/ml LPS for 4 hours, then added 300 ng/ml BFA for another 20 hours whole cell lysate at 10 µg

    Lane 2: HUVEC (human umbilical vein endothelial cell) treated with 0.5 μg/ml LPS for 4 hours, then added 300 ng/ml BFA for another 20 hours whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 11 kDa

    Observed band size: 11 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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