AB89318

Anti-CXCL1/GRO alpha antibody [MM0208-9A18]

Name: Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (ab89318) | Abcam
Brand: Abcam Limited
SKU: AB89318
Price: 525 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal CXCL1/GRO alpha antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL1.

View Alternative Names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (AB89318)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (AB89318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4μm) labelling CXCL1/GRO alpha with ab89318. Tissue was treated with 15μg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Supplier Data

Western blot - Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (AB89318)

All lanes:

Western blot - Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (ab89318) at 1/500 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 11 kDa

Observed band size: 8 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0208-9A18

Isotype

IgG2

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CXCL1.

P09341

Specificity

Cross reacts weakly with GRO beta.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.

Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

See full target information CXCL1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7489 PubMed36470882

2022

Single-cell sequencing shows cellular heterogeneity of cutaneous lesions in lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Zheng,Zhi Hu,Xiaole Mei,Lianlian Ouyang,Yang Song,Wenhui Zhou,Yi Kong,Ruifang Wu,Shijia Rao,Hai Long,Wei Shi,Hui Jing,Shuang Lu,Haijing Wu,Sujie Jia,Qianjin Lu,Ming Zhao

Cancer science 114:504-520 PubMed36169092

2022

Hepatic stellate cell-released CXCL1 aggravates HCC malignant behaviors through the MIR4435-2HG/miR-506-3p/TGFB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaling Li,Xingwang Hu,Songman Yu,Panpan Yi,Ruochan Chen,Zebing Huang,Yan Huang,Yun Huang,Rongrong Zhou,Xuegong Fan

Cancer cell international 21:552 PubMed34670584

2021

Integrated single-cell and bulk RNA sequencing analysis identifies a cancer associated fibroblast-related signature for predicting prognosis and therapeutic responses in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zheng,Heshu Liu,Yang Ge,Xin Wang

International journal of oncology 55:684-696 PubMed31322183

2019

CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Yang,Haochen Yu,Rui Chen,Kai Tao,Lei Jian,Meixi Peng,Xiaotian Li,Manran Liu,Shengchun Liu

Oncology letters 16:3231-3240 PubMed30127919

2018

Identification of six serum antigens and autoantibodies for the detection of early stage epithelial ovarian carcinoma by bioinformatics analysis and liquid chip analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yupeng Zou,Li Li

Neoplasia (New York, N.Y.) 18:636-646 PubMed27690238

2016

CXCL1-Mediated Interaction of Cancer Cells with Tumor-Associated Macrophages and Cancer-Associated Fibroblasts Promotes Tumor Progression in Human Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Makito Miyake,Shunta Hori,Yosuke Morizawa,Yoshihiro Tatsumi,Yasushi Nakai,Satoshi Anai,Kazumasa Torimoto,Katsuya Aoki,Nobumichi Tanaka,Keiji Shimada,Noboru Konishi,Michihiro Toritsuka,Toshifumi Kishimoto,Charles J Rosser,Kiyohide Fujimoto

Laboratory investigation; a journal of technical m 93:768-78 PubMed23732813

2013

Expression of CXCL1 in human endothelial cells induces angiogenesis through the CXCR2 receptor and the ERK1/2 and EGF pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Makito Miyake,Steve Goodison,Virginia Urquidi,Evan Gomes Giacoia,Charles J Rosser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL1/GRO alpha antibody [MM0208-9A18]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (AB89318)

Western blot - Anti-CXCL1/GRO alpha antibody [MM0208-9A18] (AB89318)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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