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Rabbit Recombinant Monoclonal CXCL1/GRO alpha antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (AB247986), expandable thumbnail
  • OI-RD Scanning - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (AB247986), expandable thumbnail
  • Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (AB247986), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIP
Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Additional Targets

CXCL2, CXCL3

Alternative names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

Recommended products

Rabbit Recombinant Monoclonal CXCL1/GRO alpha antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4903
Purification technique
Affinity purification Protein A
Specificity

This antibody cross-reacts with recombinant human CXCL2 and CXCL3 protein.

Dissociation constant
7.06 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247986 is the carrier-free version of Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] ab124731.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCL1 also known as GRO alpha CXCL2 and GRO gamma refers to chemokines that are important in immune response. These chemokines are small proteins with a mass of about 8 kDa. They signal through the activation of the chemokine receptor CXCR2. CXCL1/GRO alpha CXCL2 and GRO gamma express in various tissues including epithelial cells and immune cells like neutrophils. They function by recruiting these immune cells to sites of inflammation or injury.

Biological function summary

CXCL1/GRO alpha CXCL2 and GRO gamma are involved in the recruitment and activation of neutrophils. Neutrophils are the first responders in the immune response. These chemokines play a role in angiogenesis and wound healing by stimulating the migration and proliferation of endothelial cells. They do not function as part of a larger protein complex but operate independently in signaling pathways. Their chemotactic activities are essential for proper immune surveillance and response to infections.

Pathways

These chemokines are integral to the NF-kB signaling pathway and MAPK pathway. Through these pathways they mediate inflammatory responses and cellular proliferation. CXCL1/GRO alpha and its family members interact with proteins such as CXCR2 and MAPK which help relay signals for gene expression related to inflammation and cell survival. Their interaction with these pathways highlights their role in initiating and modulating inflammatory processes.

Associated diseases and disorders

CXCL1/GRO alpha CXCL2 and GRO gamma have associations with inflammatory diseases like rheumatoid arthritis and cancer especially melanoma. In rheumatoid arthritis these chemokines contribute to the recruitment of neutrophils and other immune cells to the joints leading to chronic inflammation. In cancer particularly melanoma they can promote tumor growth by enhancing angiogenesis and suppressing immune response due to the involvement of CXCR2 signaling. Understanding these chemokines involves examining proteins like CXCR2 which play a direct role in disease progression through these inflammatory and cancer pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (ab247986), expandable thumbnail

    Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (ab247986)

    This data was developed using Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] ab124731, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] ab124731) at 1/10000 dilution

    All lanes: CXCL1/GRO alpha recombinant protein at 0.005 µg

  • OI-RD Scanning - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (ab247986), expandable thumbnail

    OI-RD Scanning - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (ab247986)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (ab247986), expandable thumbnail

    Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] - BSA and Azide free (ab247986)

    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] ab124731, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] ab124731) at 1/1000 dilution

    Lane 1: His-tagged Human CXCL1(GRO alpha) recombinant protein (aa35-107) at 10 ng

    Lane 2: His-tagged Human CXCL2(GRO beta) recombinant protein (aa35-107) at 10 ng

    Lane 3: His-tagged Human CXCL3(GRO gamma) recombinant protein (aa35-107) at 50 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 7s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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