Anti-CXCL11 antibody [EPR21755-173]

• IP

Supplier Data

Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (AB216157)

CXCL11 was immunoprecipitated from 0.35mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab216157 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216157 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.

Lane 1 : THP-1 (human monocytic leukemia cell line) treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate 10ug (Input).
Lane 2 : ab216157 IP in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab216157 in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

Predicted band size: 10 kDa

Observed band size: 11 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-CXCL11 antibody [EPR21755-173] (AB216157)

Blocking/Dilution : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 17142784).

All lanes:

Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution

Lane 1:

THP-1 (hman monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 2:

THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, <a href='/en-us/products/proteins-peptides/recombinant-human-interferon-gamma-protein-active-ab9659'>ab9659</a>) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 11 kDa

true

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-CXCL11 antibody [EPR21755-173] (AB216157)

Blocking/Dilution : 5% NFDM/TBST

All lanes:

Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution

All lanes:

Human CXCL11 recombinant protein (aa 22-94) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 10 kDa

Observed band size: 9 kDa

true

Exposure time: 15s