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Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-CXCL11 antibody [EPR21755-173] (AB216157), expandable thumbnail
  • Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (AB216157), expandable thumbnail
  • Western blot - Anti-CXCL11 antibody [EPR21755-173] (AB216157), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.

Alternative names

ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC

Recommended products

Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21755-173
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCL11 also known as I-TAC or Interferon-inducible T-cell alpha chemoattractant is a small cytokine belonging to the CXC chemokine family. It weighs around 8 to 11 kilodaltons. CXCL11 predominantly expresses itself in tissues such as the thymus spleen and peripheral blood leukocytes after stimulation with interferons. Its primary role is attracting activated T-cells especially those expressing CXCR3 receptor contributing to immune surveillance and response.

Biological function summary

The chemokine plays an important role in immune system regulation and inflammation response. It attracts and activates leukocytes including T-cells and natural killer cells driving them to sites of inflammation and injury. CXCL11 is not part of a complex but interacts with other chemokines and cytokines to modulate immune responses. Its expression is upregulated in response to IFN-γ working synergistically with other chemokines like CXCL9 and CXCL10 in inflammatory environments.

Pathways

CXCL11 is integral to the chemotaxis signaling pathway. It influences the migration and activation of immune cells by interacting with the CXCR3 receptor. This pathway shares involvement with proteins such as CXCL10 which also binds to CXCR3 enabling precise directionality and regulation of immune cell movement towards inflammation sites. Additionally CXCL11's participation in the inflammatory response pathway connects it to multiple cellular responses necessary for combating infections.

Associated diseases and disorders

CXCL11 has been linked to autoimmune diseases and cancer. Its elevated expression is associated with conditions like multiple sclerosis where it may contribute to the pathological migration of immune cells into the central nervous system. In cancer CXCL11 can affect tumor microenvironments by recruiting immune cells with cytotoxic potential although this may also facilitate tumor progression in certain contexts. Proteins related to its disease association include CXCR3 and CXCL10 which together modulate various immunological outcomes in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157), expandable thumbnail

    Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

    Blocking/Dilution: 5% NFDM/TBST

    All lanes: Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution

    All lanes: Human CXCL11 recombinant protein (aa 22-94) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa

    Observed band size: 9 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (ab216157), expandable thumbnail

    Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

    CXCL11 was immunoprecipitated from 0.35mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab216157 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216157 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5,000 dilution.

    Lane 1: THP-1 (human monocytic leukemia cell line) treated with 200 ng/ml interferon-gamma (IFN-gamma, Recombinant human Interferon gamma protein (Active) ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate 10ug (Input).
    Lane 2: ab216157 IP in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, Recombinant human Interferon gamma protein (Active) ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab216157 in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, Recombinant human Interferon gamma protein (Active) ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

    Developed using the ECL technique.

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

    Exposure time: 30s

  • Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157), expandable thumbnail

    Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

    Blocking/Dilution: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 17142784).

    All lanes: Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution

    Lane 1: THP-1 (hman monocytic leukemia cell line) whole cell lysate at 10 µg

    Lane 2: THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, Recombinant human Interferon gamma protein (Active) ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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