Rabbit Recombinant Monoclonal CXCL11 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (AB279706), expandable thumbnail](https://content.abcam.com/products/images/cxcl11-antibody-epr23814-16-bsa-and-azide-free-ab279706--western-blot-img162.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (AB279706)")
![Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (AB279706), expandable thumbnail](https://content.abcam.com/products/images/cxcl11-antibody-epr23814-16-bsa-and-azide-free-ab279706--western-blot-img3189.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (AB279706)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
Alternative names
ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC
Recommended products
Rabbit Recombinant Monoclonal CXCL11 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23814-16
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab279706 is the carrier-free version of Anti-CXCL11 antibody [EPR23814-16] ab259863.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL11 also known as I-TAC or Interferon-inducible T-cell alpha chemoattractant is a small cytokine belonging to the CXC chemokine family. It weighs around 8 to 11 kilodaltons. CXCL11 predominantly expresses itself in tissues such as the thymus spleen and peripheral blood leukocytes after stimulation with interferons. Its primary role is attracting activated T-cells especially those expressing CXCR3 receptor contributing to immune surveillance and response.
- Biological function summary
The chemokine plays an important role in immune system regulation and inflammation response. It attracts and activates leukocytes including T-cells and natural killer cells driving them to sites of inflammation and injury. CXCL11 is not part of a complex but interacts with other chemokines and cytokines to modulate immune responses. Its expression is upregulated in response to IFN-γ working synergistically with other chemokines like CXCL9 and CXCL10 in inflammatory environments.
- Pathways
CXCL11 is integral to the chemotaxis signaling pathway. It influences the migration and activation of immune cells by interacting with the CXCR3 receptor. This pathway shares involvement with proteins such as CXCL10 which also binds to CXCR3 enabling precise directionality and regulation of immune cell movement towards inflammation sites. Additionally CXCL11's participation in the inflammatory response pathway connects it to multiple cellular responses necessary for combating infections.
- Associated diseases and disorders
CXCL11 has been linked to autoimmune diseases and cancer. Its elevated expression is associated with conditions like multiple sclerosis where it may contribute to the pathological migration of immune cells into the central nervous system. In cancer CXCL11 can affect tumor microenvironments by recruiting immune cells with cytotoxic potential although this may also facilitate tumor progression in certain contexts. Proteins related to its disease association include CXCR3 and CXCL10 which together modulate various immunological outcomes in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706), expandable thumbnail](https://content.abcam.com/products/images/cxcl11-antibody-epr23814-16-bsa-and-azide-free-ab279706--western-blot-img162.jpg "Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)")
Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)
This data was developed using 259863, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
All lanes: Western blot - Anti-CXCL11 antibody [EPR23814-16] (Anti-CXCL11 antibody [EPR23814-16] ab259863) at 1/2000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 40 µg
Lane 2: RAW264.7 treated with 200 ng/ml interferon-gamma for 4 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate at 40 µg
Lane 3: RAW264.7 whole cell lysate at 40 µg
Lane 4: RAW264.7 treated with 80 nM PMA for 24 hours, then treated with 100 ng/ml LPS for 4 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 3 hours, whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 10 kDa
Observed band size: 11 kDa
![Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706), expandable thumbnail](https://content.abcam.com/products/images/cxcl11-antibody-epr23814-16-bsa-and-azide-free-ab279706--western-blot-img3189.jpg "Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)")
Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)
This data was developed using 259863, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle.
Exposure time: 26 seconds.
All lanes: Western blot - Anti-CXCL11 antibody [EPR23814-16] (Anti-CXCL11 antibody [EPR23814-16] ab259863) at 1/2000 dilution
Lane 1: Mouse pancreas tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 10 kDa
Observed band size: 11 kDa
Downloads
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- Visit the general protocols
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