Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | |
---|---|
Human | Tested |
Mouse | Not recommended |
Transfected cell line - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/20 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC
Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
The IHC-P application was recommended based on cell pellets. For tissues, the positive staining was only observed in one case of human prostate cancer.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded Panel A untreated THP-1 cells, Panel B THP-1 cells treated with a combination of IFN-γ (200ng/ml, 24h) and LPS (50ng/ml, 24h). Panel C THP-1 cells treated with a combination of IFN-γ (200ng/ml 16h+8h), LPS (50ng/ml 16h+8h) add BFA (300ng/ml 8h). tissue labeling CXCL11 with ab322902 at 1/20 (25.85 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining on (C) THP-1 cells treated with a combination of IFN-γ, LPS add BFA; weak staining on (B) THP-1 cells treated with a combination of IFN-γ and LPS; no staining on (A) untreated THP-1 cells.
The section was incubated with ab322902 at 4°C overnight.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells transfected with a CXCL11 expression vector containing a his tag. Panel B HEK-293T cells transfected with a CXCL9 expression vector containing a his tag. Panel C HEK-293T cells transfected with a CXCL10 expression vector containing a his tag. Panel D HEK-293T cells transfected with a CXCL2 expression vector containing a his tag. Panel E HEK-293T cells transfected with empty vector containing a his tag. tissue labeling CXCL11 with ab322902 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a CXCL11 expression vector, no staining on (B) HEK-293T transfected with a CXCL9 expression vector, (C) HEK-293T transfected with a CXCL10 expression vector, (D) HEK-293T transfected with a CXCL2 expression vector, and (E) HEK-293T transfected with empty vector.
The section was incubated with ab322902 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling CXCL11 with ab322902 at 1/100 (5.17 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human prostate cancer.
The section was incubated with ab322902 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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