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Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Not recommended
Transfected cell line - Human
Tested

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/20 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.

Alternative names

ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC

Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27375-62
Purification technique
Affinity purification Protein A
Specificity

The IHC-P application was recommended based on cell pellets. For tissues, the positive staining was only observed in one case of human prostate cancer.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (ab322902), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (ab322902)

    Immunohistochemical analysis of paraffin-embedded Panel A untreated THP-1 cells, Panel B THP-1 cells treated with a combination of IFN-γ (200ng/ml, 24h) and LPS (50ng/ml, 24h). Panel C THP-1 cells treated with a combination of IFN-γ (200ng/ml 16h+8h), LPS (50ng/ml 16h+8h) add BFA (300ng/ml 8h). tissue labeling CXCL11 with ab322902 at 1/20 (25.85 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Positive staining on (C) THP-1 cells treated with a combination of IFN-γ, LPS add BFA; weak staining on (B) THP-1 cells treated with a combination of IFN-γ and LPS; no staining on (A) untreated THP-1 cells.

    The section was incubated with ab322902 at 4°C overnight.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (ab322902), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (ab322902)

    Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells transfected with a CXCL11 expression vector containing a his tag. Panel B HEK-293T cells transfected with a CXCL9 expression vector containing a his tag. Panel C HEK-293T cells transfected with a CXCL10 expression vector containing a his tag. Panel D HEK-293T cells transfected with a CXCL2 expression vector containing a his tag. Panel E HEK-293T cells transfected with empty vector containing a his tag. tissue labeling CXCL11 with ab322902 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with a CXCL11 expression vector, no staining on (B) HEK-293T transfected with a CXCL9 expression vector, (C) HEK-293T transfected with a CXCL10 expression vector, (D) HEK-293T transfected with a CXCL2 expression vector, and (E) HEK-293T transfected with empty vector.

    The section was incubated with ab322902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (ab322902), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (ab322902)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling CXCL11 with ab322902 at 1/100 (5.17 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human prostate cancer.

    The section was incubated with ab322902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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