AB227801

Anti-CXCL13 antibody

Name: Anti-CXCL13 antibody (ab227801) | Abcam
Brand: Abcam Limited
SKU: AB227801

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal BCA1 antibody. Suitable for ELISA, WB and reacts with Recombinant full length protein, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL13.

View Alternative Names

BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1

2 Images

Supplier Data

Western blot - Anti-CXCL13 antibody (AB227801)

All lanes:

Western blot - Anti-CXCL13 antibody (ab227801) at 0.5 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 40 µg

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

ELISA

Supplier Data

ELISA - Anti-CXCL13 antibody (AB227801)

ELISA using ab227801 as capture antibody at 4 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CXCL13.

O43927

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Recombinant full length protein": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>Capture antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in 0.2 mL of water

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 4% Trehalose, 0.9% Sodium chloride, 0.2% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.

Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

See full target information CXCL13

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 38: PubMed29769415

2018

Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Long Pan,Ling Wu,Li Pan,Yu-Xi Yang,Hu-Huan Li,Yan-Juan Dai,Zi-Qian He,Ling Tan,You-Guang Huang,Zong-Wu Tong,Jun-Long Liao

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB190662

Anti-CXCL13 antibody

Western blot - Anti-CXCL13 antibody (AB190662)

Primary Antibodies

AB89322

Anti-CXCL13 antibody [MM0212-12A2]

Western blot - Anti-CXCL13 antibody [MM0212-12A2] (AB89322)

Primary Antibodies

AB199043

Anti-CXCL13 antibody [EPR19259-147]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR19259-147] (AB199043)

Primary Antibodies

AB246518

Anti-CXCL13 antibody [EPR23400-92]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

Primary Antibodies

AB270408

Anti-CXCL13 antibody [EPR23400-92] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] - BSA and Azide free (AB270408)

Complementary Products

Assay Kits

AB269370

Human CXCL13 ELISA Kit (BCA-1)

Recombinant|SimpleStep

ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Primary Antibodies

AB254415

Anti-CXCR5 antibody [EPR23463-30]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] (AB254415)

Primary Antibodies

AB16702

Anti-CD23 antibody [SP23]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB75985

Anti-CD21 antibody [EP3093]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] (AB75985)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com