AB272874

Anti-CXCL13 antibody

Name: Anti-CXCL13 antibody (ab272874) | Abcam
Brand: Abcam Limited
SKU: AB272874
Price: 530 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal BCA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CXCL13.

View Alternative Names

BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-CXCL13 antibody (AB272874)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCL13 antibody (AB272874)

4% PFA-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for BCA1 using ab272874 at 1/500 dilution in ICC/IF (green).

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody (AB272874)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody (AB272874)

Paraffin-embedded human esophageal carcinoma tissue stained for BCA1 using ab272874 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody (AB272874)

Paraffin-embedded human esophageal carcinoma tissue stained for BCA1 using ab272874 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Supplier Data

Western blot - Anti-CXCL13 antibody (AB272874)

15% SDS-PAGE

All lanes:

Western blot - Anti-CXCL13 antibody (ab272874) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 60 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 60 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CXCL13. The exact immunogen used to generate this antibody is proprietary information.

O43927

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.

Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

See full target information CXCL13

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:863 PubMed40764989

2025

Enhanced homing and efficacy of HER2-CAR T cells via CXCR5/CCR6 co-expression for HER2-positive NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Hu,Chunlei Ge,Caixiu Huang,Dan He,Xiaoxuan Yao,Jiaxing Cheng,Jiyin Guo,Ke Li,Yunshan Ye,Li Li,Jianchuan Xia,Tao Li,Hong Yao

Cellular and molecular life sciences : CMLS 81:265 PubMed38880863

2024

Disturbed glycolipid metabolism activates CXCL13-CXCR5 axis in senescent TSCs to promote heterotopic ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyu Chen,Jinna Wu,Chipiu Wong,Wenjie Gao,Xiangdong Qi,Hang Zhou

Journal of orthopaedic surgery and research 19:162 PubMed38429811

2024

Correlation of serum and local CXCL13 levels with disease severity in patients with non-traumatic osteonecrosis of femoral head.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Heng Zhao,Wen-Xiu Zhu,Qing-He Ye,Peng Zhang,Biao-Fang Wei

Journal of cellular and molecular medicine 26:6066-6078 PubMed36458537

2022

Bacteroides plebeius improves muscle wasting in chronic kidney disease by modulating the gut-renal muscle axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Pei,Daoqi Zhu,Sixia Yang,Rong Hu,Fujing Wang,Jiaxing Zhang,Shihua Yan,Liliang Ju,Zhuoen He,Zhongxiao Han,Jinyue He,Yangtian Yan,Mingqing Wang,Wei Xiao,Yun Ma

Frontiers in oncology 11:711020 PubMed34621670

2021

Integration of scRNA-Seq and Bulk RNA-Seq to Analyse the Heterogeneity of Ovarian Cancer Immune Cells and Establish a Molecular Risk Model.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Liang,Jing Yu,Jian Li,Ning Li,Jing Liu,Lin Xiu,Jia Zeng,Tiantian Wang,Lingying Wu

Journal of neuroinflammation 17:335 PubMed33161894

2020

Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Shen,Yuan Zhang,Lihai Chen,Jiayue Du,Hongguang Bao,Yan Xing,Mengmeng Cai,Yanna Si

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL13 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CXCL13 antibody (AB272874)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody (AB272874)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody (AB272874)

Western blot - Anti-CXCL13 antibody (AB272874)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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