Rabbit Recombinant Monoclonal BCA1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (AB246518), expandable thumbnail](https://content.abcam.com/products/images/cxcl13-antibody-epr23400-92-ab246518--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29399.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (AB246518)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (AB246518), expandable thumbnail](https://content.abcam.com/products/images/cxcl13-antibody-epr23400-92-ab246518--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81627.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (AB246518)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (AB246518), expandable thumbnail](https://content.abcam.com/products/images/cxcl13-antibody-epr23400-92-ab246518--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135663.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (AB246518)")
Publications
- Nature communications 14:6102023Evolutionary route of nasopharyngeal carcinoma metastasis and its clinical significance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Lin,Xiao-Long Zhang,Rui You,You-Ping Liu,Hong-Min Cai,Li-Zhi Liu,Xue-Fei Liu,Xiong Zou,Yu-Long Xie,Ru-Hai Zou,Yi-Nuan Zhang,Rui Sun,Wei-Yi Feng,Hai-Yan Wang,Gui-Hua Tao,Hao-Jiang Li,Wen-Jie Huang,Chao Zhang,Pei-Yu Huang,Jin Wang,Qi Zhao,Qi Yang,Hong-Wan Zhang,Ting Liu,Hui-Feng Li,Xiao-Bing Jiang,Jun Tang,Yang-Kui Gu,Tao Yu,Zhi-Qiang Wang,Lin Feng,Tie-Bang Kang,Zhi-Xiang Zuo,Ming-Yuan ChenPubMed 36739462
- Frontiers in immunology 13:9537212022Genomic alteration of MTAP/CDKN2A predicts sarcomatoid differentiation and poor prognosis and modulates response to immune checkpoint blockade in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhao Xu,Aihetaimujiang Anwaier,Wangrui Liu,Gaomeng Wei,Jiaqi Su,Xi Tian,Jing Xia,Yuanyuan Qu,Jianyuan Zhao,Hailiang Zhang,Dingwei YePubMed 35979371
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Target data
Function
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
Alternative names
C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1, BLC, BCA1, SCYB13, CXCL13
Recommended products
Rabbit Recombinant Monoclonal BCA1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.
Alternative names
C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1, BLC, BCA1, SCYB13, CXCL13
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23400-92
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- Activity summary
CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.
- Biological function summary
In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.
- Pathways
CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.
- Associated diseases and disorders
CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518), expandable thumbnail](https://content.abcam.com/products/images/cxcl13-antibody-epr23400-92-ab246518--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29399.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CXCL13 with ab246518 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the germinal center in the human tonsil. The section was incubated with ab246518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518), expandable thumbnail](https://content.abcam.com/products/images/cxcl13-antibody-epr23400-92-ab246518--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81627.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CXCL13 with ab246518 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the lymphoid tissue in the human colon. The section was incubated with ab246518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518), expandable thumbnail](https://content.abcam.com/products/images/cxcl13-antibody-epr23400-92-ab246518--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135663.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR23400-92] (ab246518)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CXCL13 with ab246518 at 1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on the human kidney (PMID: 19955043). The section was incubated with ab246518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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