AB46010

Anti-CXCL14 antibody

Name: Anti-CXCL14 antibody (ab46010) | Abcam
Brand: Abcam Limited
SKU: AB46010

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(12 Publications)

Rabbit Polyclonal CXCL14 antibody. Suitable for ELISA, WB, sELISA, IHC-P, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL14.

View Alternative Names

MIP2G, NJAC, SCYB14, PSEC0212, UNQ240/PRO273, CXCL14, C-X-C motif chemokine 14, Chemokine BRAK, MIP-2G, Small-inducible cytokine B14

1 Images

Sandwich ELISA - Anti-CXCL14 antibody (AB46010)

sELISA

Unknown

Sandwich ELISA - Anti-CXCL14 antibody (AB46010)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ELISA, sELISA, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CXCL14.

O95715

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "Detection limit 0.2 - 0.4 ng/well.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducing conditions.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "To detect CXCL14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Although some customers have been successful in IHC we no longer batch test in this application.The sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

Purification technique

Affinity purification Protein A

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CXCL14 protein also known as BRAK is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of 8.9 kDa and is expressed in various tissues with high levels in the kidney lung skin and reproductive tissues. It does not normally circulate in the blood but rather acts in tissues. The protein plays a role in immune system regulation by being involved in the migration of immune cells.

Biological function summary

CXCL14 influences cell signaling pathways that regulate immune responses and tissue homeostasis. It engages in the attraction of immune cells like monocytes and dendritic cells to specific tissues. While CXCL14 is not part of a larger complex its function in modulating local immune environments is significant. This protein’s role in immune surveillance contributes to both normal physiological conditions and response to pathology.

Pathways

Interactions of CXCL14 involve key signaling cascades. The protein is part of pathways that regulate inflammation and cell movement including the MAPK and PI3K signaling pathways. Related proteins such as CXCR4 and CCR2 interact within these pathways to coordinate cell signaling and chemotaxis. These interactions facilitate communication between cells and regulate responses essential for maintaining tissue integrity.

CXCL14 links with conditions like cancer and obesity. Studies suggest that CXCL14 may play a dual role in cancer acting as a tumor suppressor in certain contexts by influencing immune cell infiltration into tumors. In obesity altered expression patterns of CXCL14 have been observed highlighting potential roles in adipose tissue regulation. Proteins like CXCR4 are also connected with CXCL14 in these disease contexts suggesting complex interdependencies within the cellular communication network.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.

See full target information CXCL14

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0281798 PubMed36952534

2023

A three protein signature fails to externally validate as a biomarker to predict surgical outcome in high-grade epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Amy Hawarden,Marcus Price,Bryn Russell,Godfrey Wilson,Laura Farrelly,Andrew Embleton-Thirsk,Mahesh Parmar,Richard Edmondson

Cell discovery 8:58 PubMed35725971

2022

Single-cell RNA sequencing of subcutaneous adipose tissues identifies therapeutic targets for cancer-associated lymphedema.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanyu Liu,Meng Yuan,Qinqin Xiang,Zhujun Li,Fen Xu,Wen Chen,Jie Chen,Jiuzuo Huang,Nanze Yu,Zhou Zhou,Xiao Long

Oncogenesis 10:66 PubMed34611125

2021

Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xu,Runtian Wang,Hui Xie,Longfei Hu,Cong Wang,Jiali Xu,Chengjun Zhu,Yiqiu Liu,Fangyan Gao,Xintong Li,Cenzhu Wang,Jinyi Huang,Wenbin Zhou,Guohua Zhou,Yongqian Shu,Xiaoxiang Guan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:11257-11271 PubMed32648265

2020

Two coupled mutations abolished the binding of CEBPB to the promoter of CXCL14 that displayed an antiviral effect on PRRSV by activating IFN signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhu Niu,Zhiwei Zheng,Qianjing Xue,Huijun Cheng,Ying Liu,Huanling Wang,Xueying Hu,Anding Zhang,Bang Liu,Xuewen Xu

Cancer science 111:2588-2597 PubMed32403160

2020

High expression of CXCL14 is a biomarker of lung adenocarcinoma with micropapillary pattern.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Sata,Takahiro Nakajima,Masaki Fukuyo,Keisuke Matsusaka,Atsushi Hata,Junichi Morimoto,Bahityar Rahmutulla,Yuki Ito,Hidemi Suzuki,Ichiro Yoshino,Atsushi Kaneda

The Journal of clinical investigation 128:5235-5250 PubMed30204129

2018

HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Wang,Xiaoling Weng,Luoyang Wang,Mingang Hao,Yue Li,Lidan Hou,Yu Liang,Tianqi Wu,Mengfei Yao,Guowen Lin,Yiwei Jiang,Guohui Fu,Zhaoyuan Hou,Xiangjun Meng,Jinsong Lu,Jianhua Wang

Medicine 97:e0101 PubMed29517684

2018

CXCL14 and NOS1 expression in specimens from patients with stage I-IIIA nonsmall cell lung cancer after curative resection.

Applications

IHC

Species

Human

Xiaoqin Ji,Zetian Shen,Benxin Zhao,Xi Yuan,Xixu Zhu

Oncotarget 7:14207-19 PubMed26893359

2016

Transcriptomic analysis of stage 1 versus advanced adult granulosa cell tumors.

Applications

IHC

Species

Unspecified reactive species

Maria Alexiadis,Simon Chu,Dilys Leung,Jodee A Gould,Tom Jobling,Peter J Fuller

Journal of the National Cancer Institute 106: PubMed24700803

2014

Risk prediction for late-stage ovarian cancer by meta-analysis of 1525 patient samples.

Applications

IHC-P

Species

Human

Markus Riester,Wei Wei,Levi Waldron,Aedin C Culhane,Lorenzo Trippa,Esther Oliva,Sung-Hoon Kim,Franziska Michor,Curtis Huttenhower,Giovanni Parmigiani,Michael J Birrer

Cancer science 104:1523-31 PubMed24033560

2013

Antitumor efficacy of C-X-C motif chemokine ligand 14 in hepatocellular carcinoma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Wang,Pengfei Huang,Lufei Zhang,Jianfeng Wei,Qingsong Xie,Qiang Sun,Xiaohu Zhou,Haiyang Xie,Lin Zhou,Shusen Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL14 antibody

Sandwich ELISA - Anti-CXCL14 antibody (AB46010)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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