Anti-CXCL14 antibody [EPR22807-28]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CXCL14 antibody [EPR22807-28] (AB264467)

Intracellular flow cytometric analysis of [4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/500 dilution (Right) compared with a Isotype control details (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150079), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab264467 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor^® 488.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (AB264467)

Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). The myc tag is also detected with a Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 647 Conjugate) (Red). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged CXCL14 expression vector. The nuclear counter stain is DAPI (Blue).
Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution.

• WB

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Western blot - Anti-CXCL14 antibody [EPR22807-28] (AB264467)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 15843547, 2602137, 28359053).

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

Rat skin lysate at 10 µg

Lane 3:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

Mouse skin lysate at 20 µg

Lane 5:

Rat lung lysate at 20 µg

Lane 6:

Mouse lung lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 3 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 14 kDa

false

• WB

Unknown

Western blot - Anti-CXCL14 antibody [EPR22807-28] (AB264467)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with CXCL14 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

false