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Rabbit Recombinant Monoclonal CXCL15 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.

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Images

Western blot - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227), expandable thumbnail
  • Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Mouse
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions.

Alternative names

Scyb15, Cxcl15, C-X-C motif chemokine 15, Lungkine, Small-inducible cytokine B15

Recommended products

Rabbit Recombinant Monoclonal CXCL15 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14470
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251227 is the carrier-free version of Anti-CXCL15 antibody [EPR14470] ab197016.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCL15 also known as lungkine is a small cytokine belonging to the CXC chemokine family. This protein exhibits a molecular mass of approximately 9 kDa. CXCL15 is expressed mainly in epithelial cells of the lung where it facilitates the recruitment of neutrophils. Unlike some other cytokines lungkine is produced constitutively and does not require induction by inflammatory stimuli. Its role as a chemokine indicates a specific involvement in respiratory immune responses.

Biological function summary

CXCL15 is involved in the recruitment and activation of neutrophils playing a pivotal role in the innate immune defense. It does not form a complex; instead it functions as a soluble signal guiding the migration of immune cells to sites of inflammation in the lungs. CXCL15 contributes to the body's defense mechanisms against infection. Its interaction with neutrophils is important for maintaining pulmonary health and responding to pathogenic challenges.

Pathways

The recruitment activity of CXCL15 is involved in chemokine signaling and immune response pathways. CXCL15 interacts with G protein-coupled receptors on neutrophils to drive chemotaxis the directed movement of cells toward areas of infection. This pathway involves cooperation with other cytokines such as CXCL1 and CXCL8 which also play roles in neutrophil trafficking and activation in inflammatory scenarios.

Associated diseases and disorders

CXCL15 has a relationship with chronic obstructive pulmonary disease (COPD) and acute lung injury. Elevated levels of CXCL15 may exacerbate inflammatory conditions contributing to the progression and severity of these diseases. In COPD CXCL15 influences neutrophil accumulation in the airways often linked with other chemokines like IL-8. Understanding its role may offer insight into therapeutic strategies targeting excessive neutrophilic infiltration in lung-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227), expandable thumbnail

    Western blot - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227)

    This data was developed using Anti-CXCL15 antibody [EPR14470] ab197016, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CXCL15 antibody [EPR14470] (Anti-CXCL15 antibody [EPR14470] ab197016) at 1/5000 dilution

    Lane 1: Mouse lung tissue lysate at 10 µg

    Lane 2: Mouse brain tissue lysate at 10 µg

    Lane 3: Mouse heart tissue lysate at 10 µg

    Lane 4: Mouse kidney tissue lysate at 10 µg

    Lane 5: Mouse spleen tissue lysate at 10 µg

    Lane 6: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 7: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227)

    This data was developed using Anti-CXCL15 antibody [EPR14470] ab197016, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL15 with Anti-CXCL15 antibody [EPR14470] ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227), expandable thumbnail

    Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227)

    This data was developed using Anti-CXCL15 antibody [EPR14470] ab197016, the same antibody clone in a different buffer formulation.CXCL15 was immunoprecipitated from Mouse lung lysates with Anti-CXCL15 antibody [EPR14470] ab197016 at 1/70 dilution. Western blot was performed from the immunoprecipitate using Anti-CXCL15 antibody [EPR14470] ab197016 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Mouse lung lysate 10 µg (Input). Lane 2: Anti-CXCL15 antibody [EPR14470] ab197016 IP in Mouse lung lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CXCL15 antibody [EPR14470] ab197016 in Mouse lung lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] (Anti-CXCL15 antibody [EPR14470] ab197016)

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (ab251227)

    This data was developed using Anti-CXCL15 antibody [EPR14470] ab197016, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CXCL15 with Anti-CXCL15 antibody [EPR14470] ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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