Anti-CXCL15 antibody [EPR14470] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227)

This data was developed using ab197016, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227)

This data was developed using ab197016, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227)

This data was developed using ab197016, the same antibody clone in a different buffer formulation.

CXCL15 was immunoprecipitated from Mouse lung lysates with ab197016 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197016 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Mouse lung lysate 10 μg (Input). Lane 2 : ab197016 IP in Mouse lung lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab197016 in Mouse lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] (<a href='/en-us/products/primary-antibodies/cxcl15-antibody-epr14470-ab197016'>ab197016</a>)

Predicted band size: 19 kDa

false

• WB

Supplier Data

Western blot - Anti-CXCL15 antibody [EPR14470] - BSA and Azide free (AB251227)

This data was developed using ab197016, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CXCL15 antibody [EPR14470] (<a href='/en-us/products/primary-antibodies/cxcl15-antibody-epr14470-ab197016'>ab197016</a>) at 1/5000 dilution

Lane 1:

Mouse lung tissue lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Lane 4:

Mouse kidney tissue lysate at 10 µg

Lane 5:

Mouse spleen tissue lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 7:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false