Skip to main content

Anti-CXCL16 antibody [EPR25370-42] is a Rabbit Monoclonal antibody that is used in CXCL16 IHC-P, Western Blot. Suitable for Human samples.

CXCL16 is a chemokine known for its ability to attract immune cells, such as T cells and natural killer (NK) cells, into the tumor microenvironment. This recruitment can enhance anti-tumor immunity.The expression of CXCL16 can influence tumor cell proliferation, migration, invasion, and metastasis. It has been observed in various cancers, including breast, prostate, and colorectal cancers.CXCL16 affects the tumor microenvironment by interacting with different cell types, such as tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), and regulatory T cells (Tregs). These interactions can either promote or inhibit tumor growth depending on the context.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CXCL16 antibody [EPR25370-42] (AB307694), expandable thumbnail
  • Western blot - Anti-CXCL16 antibody [EPR25370-42] (AB307694), expandable thumbnail
  • Western blot - Anti-CXCL16 antibody [EPR25370-42] (AB307694), expandable thumbnail
  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694), expandable thumbnail
  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow CytFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

CXCL16 undergoes cleavage and glycosylation modifications. Consequently, different band sizes corresponding to its distinct forms can be detected: the 28 kDa precursor form, the 40-50 kDa mature form, and the 35 kDa soluble form. Please refer to publications such as PMID: 39436058, PMID: 17467666, and PMID: 33800554.

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Target data

Function

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Alternative names

SCYB16, SRPSOX, UNQ2759/PRO6714, CXCL16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX

Recommended products

Anti-CXCL16 antibody [EPR25370-42] is a Rabbit Monoclonal antibody that is used in CXCL16 IHC-P, Western Blot. Suitable for Human samples.

CXCL16 is a chemokine known for its ability to attract immune cells, such as T cells and natural killer (NK) cells, into the tumor microenvironment. This recruitment can enhance anti-tumor immunity.The expression of CXCL16 can influence tumor cell proliferation, migration, invasion, and metastasis. It has been observed in various cancers, including breast, prostate, and colorectal cancers.CXCL16 affects the tumor microenvironment by interacting with different cell types, such as tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), and regulatory T cells (Tregs). These interactions can either promote or inhibit tumor growth depending on the context.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25370-42
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.

Biological function summary

CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.

Pathways

CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.

Associated diseases and disorders

CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    CXCL16 Western blot staining using rabbit Anti-CXCL16 antibody

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.

    CXCL16 undergoes cleavage and glycosylation modifications. Consequently, different band sizes corresponding to its distinct forms can be detected: the 28 kDa precursor form, the 40-50 kDa mature form, and the 35 kDa soluble form. Please refer to publications such as PMID: 39436058, PMID: 17467666, and PMID: 33800554.

    All lanes: Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) treated with 1000U/ml IFN gamma for 18 hours whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) cell culture supernatant lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 35 kDa, 50 kDa

    Exposure time: 180s

  • Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    CXCL16 Western blot staining using rabbit Anti-CXCL16 antibody

    CXCL16 undergoes cleavage and glycosylation modifications. Consequently, different band sizes corresponding to its distinct forms can be detected: the 28 kDa precursor form, the 40-50 kDa mature form, and the 35 kDa soluble form. Please refer to publications such as PMID: 39436058, PMID: 17467666, and PMID: 33800554.

    All lanes: Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694) at 1/1000 dilution

    Lane 1: Human lymph node tissue lysate at 20 µg

    Lane 2: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 28 kDa

    Exposure time: 180s

  • Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: skeletal muscle.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Observed band size: 30 kDa

    Exposure time: 180s

  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    Immunohistochemical analysis of paraffin-embedded cells labeling CXCL16 with ab307694 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in (A) HEK293T cells co-transfected with a CXCL16 expression vector containing a his tag and no staining in (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on immune cells of human colon. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human testis. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694), expandable thumbnail

    Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (ab307694)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human skeletal muscle. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com