Anti-CXCL16 antibody [EPR25370-42]

• IHC

Supplier Data

Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on human skeletal muscle. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

Immunohistochemical analysis of paraffin-embedded cells labeling CXCL16 with ab307694 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in (A) HEK293T cells co-transfected with a CXCL16 expression vector containing a his tag and no staining in (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CXCL16 with ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on immune cells of human colon. The section was incubated with ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 30 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

CXCL16 undergoes cleavage and glycosylation modifications. Consequently, different band sizes corresponding to its distinct forms can be detected : the 28 kDa precursor form, the 40-50 kDa mature form, and the 35 kDa soluble form. Please refer to publications such as PMID : 39436058, PMID : 17467666, and PMID : 33800554.

All lanes:

Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694) at 1/1000 dilution

Lane 1:

Human lymph node tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 28 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-CXCL16 antibody [EPR25370-42] (AB307694)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

CXCL16 undergoes cleavage and glycosylation modifications. Consequently, different band sizes corresponding to its distinct forms can be detected : the 28 kDa precursor form, the 40-50 kDa mature form, and the 35 kDa soluble form. Please refer to publications such as PMID : 39436058, PMID : 17467666, and PMID : 33800554.

All lanes:

Western blot - Anti-CXCL16 antibody [EPR25370-42] (ab307694) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) treated with 1000U/ml IFN gamma for 18 hours whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) cell culture supernatant lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35 kDa,50 kDa

false

Exposure time: 180s