Rabbit Recombinant Monoclonal CXCL16 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--western-blot-img408983.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695)")
![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408467.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695)")
![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408466.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695)")
![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408465.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695)")
![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408464.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (AB307695)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.
Alternative names
SCYB16, SRPSOX, UNQ2759/PRO6714, CXCL16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX
Recommended products
Rabbit Recombinant Monoclonal CXCL16 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25370-42
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.
- Biological function summary
CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.
- Pathways
CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.
- Associated diseases and disorders
CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--western-blot-img408983.jpg "Western blot - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
Western blot - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)
This data was produced using Anti-CXCL16 antibody [EPR25370-42] ab307694, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-CXCL16 antibody [EPR25370-42] (Anti-CXCL16 antibody [EPR25370-42] ab307694) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 30 kDa
Exposure time: 180s
![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408467.jpg "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)
This data was produced using Anti-CXCL16 antibody [EPR25370-42] ab307694, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded cells labeling CXCL16 with Anti-CXCL16 antibody [EPR25370-42] ab307694 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in (A) HEK293T cells co-transfected with a CXCL16 expression vector containing a his tag and no staining in (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with Anti-CXCL16 antibody [EPR25370-42] ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408466.jpg "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)
This data was produced using Anti-CXCL16 antibody [EPR25370-42] ab307694, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CXCL16 with Anti-CXCL16 antibody [EPR25370-42] ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with Anti-CXCL16 antibody [EPR25370-42] ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408465.jpg "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)
This data was produced using Anti-CXCL16 antibody [EPR25370-42] ab307694, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CXCL16 with Anti-CXCL16 antibody [EPR25370-42] ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on immune cells of human colon. The section was incubated with Anti-CXCL16 antibody [EPR25370-42] ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408464.jpg "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)
This data was produced using Anti-CXCL16 antibody [EPR25370-42] ab307694, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling CXCL16 with Anti-CXCL16 antibody [EPR25370-42] ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human testis. The section was incubated with Anti-CXCL16 antibody [EPR25370-42] ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408463.jpg "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)
This data was produced using Anti-CXCL16 antibody [EPR25370-42] ab307694, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling CXCL16 with Anti-CXCL16 antibody [EPR25370-42] ab307694 at 1/200 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human skeletal muscle. The section was incubated with Anti-CXCL16 antibody [EPR25370-42] ab307694 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695), expandable thumbnail](https://content.abcam.com/products/images/cxcl16-antibody-epr25370-42-bsa-and-azide-free-ab307695--immunohistochemistry-img408463.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-CXCL16 antibody [EPR25370-42] - BSA and Azide free (ab307695)")
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