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AB322709

Anti-CXCL16 antibody [EPR29713-506]

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Rabbit Recombinant Monoclonal CXCL16 antibody. Suitable for Dot, Flow Cyt and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

SCYB16, SRPSOX, UNQ2759/PRO6714, CXCL16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX

3 Images
Flow Cytometry - Anti-CXCL16 antibody [EPR29713-506] (AB322709)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CXCL16 antibody [EPR29713-506] (AB322709)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CXCL16 with ab322709 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC were co-stained with CD19 conjugated Alexa Fluor®647.
Gated on viable cell.

Flow Cytometry - Anti-CXCL16 antibody [EPR29713-506] (AB322709)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CXCL16 antibody [EPR29713-506] (AB322709)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CXCL16 with ab322709 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC were co-stained with CD11b conjugated BV421.
Gated on viable cell.

Dot Blot - Anti-CXCL16 antibody [EPR29713-506] (AB322709)
  • Dot

Supplier Data

Dot Blot - Anti-CXCL16 antibody [EPR29713-506] (AB322709)

Dot blot analysis of CXCL16 using ab322709 at 1 : 1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : Human CXCL16 protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CXCL16 antibody [EPR29713-506] (ab322709) at 1/1000 dilution

All lanes:

Human CXCL16 protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CXCL16 antibody [EPR29713-506]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CXCL16 antibody [EPR29713-506]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CXCL16 antibody [EPR29713-506]

  • 578 PE

    PE Anti-CXCL16 antibody [EPR29713-506]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29713-506

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.
See full target information CXCL16
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Complementary Products

Assay Kits

AB187397

Human CXCL16 ELISA Kit

SimpleStep

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com