AB9777

Anti-CXCL2 antibody

Name: Anti-CXCL2 antibody (ab9777) | Abcam
Brand: Abcam Limited
SKU: AB9777
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal CXCL2 antibody. Suitable for ELISA, WB, sELISA, Nucleotide Array, IHC-Fr and reacts with Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Cxcl2.

View Alternative Names

Cinc3, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2, CINC-3, MIP2

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Reacts with

Rat

Applications

sELISA, Nucleotide Array, ELISA, WB, IHC-Fr

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Cxcl2.

P30348

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "NucleotideArray" : {"fullname" : "Nucleotide Array", "shortname":"Nucleotide Array"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "To detect Rat CXCL2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of ab9777 is required. This antigen affinity purified antibody, in conjunction with an Anti-Rat CXCL2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CXCL2.", "NucleotideArray-species-checked": "guaranteed", "NucleotideArray-species-dilution-info": "", "NucleotideArray-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

Product details

Although some customers have been successful in IHC we no longer batch test in this application.

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.

Biological function summary

CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.

Pathways

CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).

CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.

See full target information Cxcl2

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Annals of neurology 91:532-547 PubMed35167145

2022

RGMa Signal in Macrophages Induces Neutrophil-Related Astrocytopathy in NMO.

Applications

Unspecified application

Species

Unspecified reactive species

Shosuke Iwamoto,Takahide Itokazu,Atsushi Sasaki,Hirotoshi Kataoka,Shinji Tanaka,Takeshi Hirata,Keiko Miwa,Toshihiko Suenaga,Yoshiki Takai,Tatsuro Misu,Kazuo Fujihara,Toshihide Yamashita

Toxins 12: PubMed32438602

2020

Classical and Alternative Activation of Rat Microglia Treated with Ultrapure Lipopolysaccharide In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Zylfi Memedovski,Evan Czerwonka,Jin Han,Joshua Mayer,Margaret Luce,Lucas C Klemm,Mary L Hall,Alejandro M S Mayer

Neural regeneration research 15:2154-2161 PubMed32394974

2020

Knocking down TRPM2 expression reduces cell injury and NLRP3 inflammasome activation in PC12 cells subjected to oxygen-glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Pan,Qiu-Jiao Zhu,Li-Xiao Xu,Xin Ding,Jian-Qin Li,Bin Sun,Jun Hua,Xing Feng

Stem cell research & therapy 8:214 PubMed28962647

2017

miR-524-5p of the primate-specific C19MC miRNA cluster targets TP53IPN1- and EMT-associated genes to regulate cellular reprogramming.

Applications

Species

Unspecified reactive species

Phan Nguyen Nhi Nguyen,Kong Bung Choo,Chiu-Jung Huang,Shigeki Sugii,Soon Keng Cheong,Tunku Kamarul

Nature communications 6:8017 PubMed26281983

2015

CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Yu,Shu-Yan Ji,Qian-Qian Sha,Qing-Yuan Sun,Heng-Yu Fan

PloS one 8:e66432 PubMed23776670

2013

Chemokine localization in bronchial angiogenesis.

Applications

IHC-Fr

Species

Rat

Maria Grazia Perino,Aigul Moldobaeva,John Jenkins,Elizabeth M Wagner

Journal of inflammation (London, England) 6:27 PubMed19781090

2009

Homocysteine-induced macrophage inflammatory protein-2 production by glomerular mesangial cells is mediated by PI3 Kinase and p38 MAPK.

Applications

Species

Rat

Suresh Shastry,Leighton R James

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL2 antibody

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise