Rabbit Recombinant Monoclonal CXCL2 antibody. Suitable for IP, WB and reacts with Rat samples. Cited in 2 publications.
Images
![Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (AB275879), expandable thumbnail](https://content.abcam.com/products/images/cxcl2-antibody-epr23331-159-ab275879--immunoprecipitation-img9903.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (AB275879)")
![Western blot - Anti-CXCL2 antibody [EPR23331-159] (AB275879), expandable thumbnail](https://content.abcam.com/products/images/cxcl2-antibody-epr23331-159-ab275879--western-blot-img28946.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL2 antibody [EPR23331-159] (AB275879)")
![Western blot - Anti-CXCL2 antibody [EPR23331-159] (AB275879), expandable thumbnail](https://content.abcam.com/products/images/cxcl2-antibody-epr23331-159-ab275879--western-blot-img11457.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL2 antibody [EPR23331-159] (AB275879)")
Publications
Filter by
- Heliyon 9:e153052023EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Jie Zhang,Zhi-Xiang Sun,Hai-Jian LiuPubMed 37101626
- International heart journal 63:755-7622022Ursolic Acid Ameliorates the Injury of H9c2 Cells Caused by Hypoxia and Reoxygenation Through Mediating CXCL2/NF-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongrui Bian,Fei Xu,Hui Liu,Yimeng DuPubMed 35831147
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Alternative names
Cinc3, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2, CINC-3, MIP2
Recommended products
Rabbit Recombinant Monoclonal CXCL2 antibody. Suitable for IP, WB and reacts with Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23331-159
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
- Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
- Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
- Associated diseases and disorders
CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (ab275879), expandable thumbnail](https://content.abcam.com/products/images/cxcl2-antibody-epr23331-159-ab275879--immunoprecipitation-img9903.jpg "Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (ab275879)")
Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
This data was developed using ab275879, the same antibody clone in a different buffer formulation.CXCL2 was immunoprecipitated from 0.35 mg Rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant 10 ?l with ab275879 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275879 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant 10 ?l
Lane 2: abab275879 IP in Rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275879 in rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
Predicted band size: 11 kDa
Observed band size: 10 kDa
![Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879), expandable thumbnail](https://content.abcam.com/products/images/cxcl2-antibody-epr23331-159-ab275879--western-blot-img28946.jpg "Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)")
Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution
Lane 1: Untreated rat lung organ culture, supernatant at 10 µL
Lane 2: Rat lung organ (treated with 10/ml Concanavalin A for 3 days) culture, supernatant at 10 µL
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
![Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879), expandable thumbnail](https://content.abcam.com/products/images/cxcl2-antibody-epr23331-159-ab275879--western-blot-img11457.jpg "Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)")
Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
All lanes: Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution
All lanes: Recombinant Rat CXCL2 protein, 10 ng
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
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