Anti-CXCL5 antibody [RP23040004]

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-CXCL5 antibody [RP23040004] (AB308023)

Immunohistochemical analysis of paraffin-embedded human brain tissue labelling CXCL5 with ab308023 followed by HRP-conjugated secondary antibody and colorimetric detection using a DAB kit. (right) and without ab308023 (left). The section was incubated with ab308023 at 1/20 dilution overnight at 4ºC in a humidified chamber. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-CXCL5 antibody [RP23040004] (AB308023)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling CXCL5 with ab308023 diluted in 3% BSA-PBS at a 1/100 dilution followed by HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. (right) and without ab308023 (left). The section was incubated with ab308023 overnight at 4ºC in a humidified chamber. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS.

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-CXCL5 antibody [RP23040004] (AB308023)

Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labelling CXCL5 with ab308023 diluted in 3% BSA-PBS at a 1/50 dilution followed by HRP-conjugated secondary antibody and detection using a DAB kit. (right) and without ab308023 (left). The section was incubated with ab308023 at 1/50 dilution overnight at 4ºC in a humidified chamber. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS

• WB

Supplier Data

Western blot - Anti-CXCL5 antibody [RP23040004] (AB308023)

Blocking and dilution buffer : 5% skim milk

CXCL5 was detected at ~ 8 kDa using ab308023 Anti-CXCL5 antibody [RP23040004] at 1-3 μg/mL in 5 % skim milk for 3 hours at room temperature on a rocking platform

All lanes:

Western blot - Anti-CXCL5 antibody [RP23040004] (ab308023)

Lane 1:

Human A549 cell lysate at 30 µg

Lane 2:

Human A549 treated for 20 min with 200 nM of PMA cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG - HRP at 1/5000 dilution

Predicted band size: 12 kDa

Observed band size: 8 kDa

false

• WB

Supplier Data

Western blot - Anti-CXCL5 antibody [RP23040004] (AB308023)

Western blot analysis of CXCL5 in A549 cell lysate (30 µg/lane) using ab308023 at a dilution of 2 µg/mL. NBT/BCIP was used as the substrate.

All lanes:

Western blot - Anti-CXCL5 antibody [RP23040004] (ab308023) at 2 µg/mL

All lanes:

A549 cell lysate at 30 µg

Observed band size: 8 kDa

true