Rabbit Monoclonal CXCL5 antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 7 publications.
Images
![Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (AB198505), expandable thumbnail](https://content.abcam.com/products/images/cxcl5-cxcl6-antibody-ep13083-ab198505--immunoprecipitation-img429951.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (AB198505)")
![Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (AB198505), expandable thumbnail](https://content.abcam.com/products/images/cxcl5-cxcl6-antibody-ep13083-ab198505--western-blot-img429950.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (AB198505)")
![Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (AB198505), expandable thumbnail](https://content.abcam.com/products/images/cxcl5-cxcl6-antibody-ep13083-ab198505--western-blot-img429949.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (AB198505)")
Publications
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- Cell reports 41:1118482022IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanxi Xiao,Rosie Kumar,Yutaro Komuro,Jasmine Burguet,Visesha Kakarla,Ida Azizkhanian,Sunil A Sheth,Christopher K Williams,Xinhai R Zhang,Michal Macknicki,Andrew Brumm,Riki Kawaguchi,Phu Mai,Naoki Kaneko,Harry V Vinters,S Thomas Carmichael,Leif A Havton,Charles DeCarli,Jason D HinmanPubMed 36543124
- The Journal of international medical research 49:3000605219977182021Expression and potential role of CXCL5 in the pathogenesis of intrauterine adhesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Ang Fang,Yu He,Chao Sun,Lei Zhan,Guiju Zhou,Bing Wei,Shiying SunPubMed 33752504
- Cell stem cell 25:797-813.e92019Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Wilder Scott,Martin Arostegui,Ronen Schweitzer,Fabio M V Rossi,T Michael UnderhillPubMed 31809738
- Frontiers in pharmacology 10:3072019Activation of CXCL6/CXCR1/2 Axis Promotes the Growth and Metastasis of Osteosarcoma Cells and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangchen Liu,Liping An,Hongmei Zhang,Peige Du,Yu ShengPubMed 30984000
- Autophagy 15:1356-13752019SIRT3 promotes antimycobacterial defenses by coordinating mitochondrial and autophagic functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae Sung Kim et. al.PubMed 30774023
- Immunity 49:764-779.e92018The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGurkan Mollaoglu,Alex Jones,Sarah J Wait,Anandaroop Mukhopadhyay,Sangmin Jeong,Rahul Arya,Soledad A Camolotto,Timothy L Mosbruger,Chris J Stubben,Christopher J Conley,Arjun Bhutkar,Jeffery M Vahrenkamp,Kristofer C Berrett,Melissa H Cessna,Thomas E Lane,Benjamin L Witt,Mohamed E Salama,Jason Gertz,Kevin B Jones,Eric L Snyder,Trudy G OliverPubMed 30332632
- Scientific reports 7:139882017Transcriptome Analysis Reveals Neuroprotective aspects of Human Reactive Astrocytes induced by Interleukin 1β.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Boon Loong Teh et. al.PubMed 29070875
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
Additional Targets
CXCL6
Alternative names
ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5
Recommended products
Rabbit Monoclonal CXCL5 antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP13083
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The production method for this product has been changed from hybridoma to recombinant on 15th April 2024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-X-C motif chemokine ligands 5 and 6 (CXCL5 and CXCL6) also known as ENA-78 and GCP-2 respectively are small proteins with a molecular mass of approximately 12 kDa. These chemokines belong to the CXC chemokine family and play key roles as signaling proteins. CXCL5 and CXCL6 are mainly expressed in neutrophils epithelial cells and to some extent in macrophages. When they bind to their receptor CXCR2 they induce chemotaxis guiding immune cells toward sites of inflammation or injury.
- Biological function summary
They coordinate immune response by mediating neutrophil recruitment to inflammatory sites. CXCL5 and CXCL6 often work as a team binding CXCR2 and aiding in protective tissue responses. Their roles extend further as they partake in triggering angiogenesis the formation of new blood vessels under physiological or pathological conditions such as wound healing or cancer. These activities imply their involvement in complex cellular networks and interactions.
- Pathways
The involvement of CXCL5 and CXCL6 arises prominently in the NF-kB pathway and the MAPK signaling pathway. These pathways integral to immune and inflammatory responses reflect the chemokines' interaction with proteins like IL-8 and TNF-alpha emphasizing their importance in host defense mechanisms and inflammatory signaling cascades. Their ability to engage with these pathways demonstrates an essential role in modulating the intensity and duration of inflammatory responses.
- Associated diseases and disorders
CXCL5 and CXCL6 relate to conditions such as cancer and rheumatoid arthritis. In cancer these chemokines can enhance tumor progression by increasing angiogenesis and attracting cells that promote tumor growth. Similarly in rheumatoid arthritis their overexpression correlates with joint inflammation and tissue damage. Proteins like VEGF-A connected to these chemokines further underline their association with pathological angiogenesis and inflammatory process regulation in disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505), expandable thumbnail](https://content.abcam.com/products/images/cxcl5-cxcl6-antibody-ep13083-ab198505--immunoprecipitation-img429951.jpg "Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)")
Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)
CXCL5 + CXCL6 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell extract with ab198505 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab198505 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: A549 (Human lung carcinoma epithelial cell), whole cell lysate, 10µg
Lane 2: A549 whole cell lysate with the ab198505 at 1/30 dilution
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab198505 in A549 whole cell lysateAll lanes: Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/1000 dilution
All lanes: A549 (Human lung carcinoma epithelial cell), whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 12 kDa
Exposure time: 93s
![Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505), expandable thumbnail](https://content.abcam.com/products/images/cxcl5-cxcl6-antibody-ep13083-ab198505--western-blot-img429950.jpg "Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)")
Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)
The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID: 23922745).
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used a GAPDH loading control.
All lanes: Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/10000 dilution
Lane 1: HEK-293T cells transfected with an empty expression vector containing His tag, hole cell lysate with 5% NFDM/TBST
Lane 2: HEK-293T cells transfected with a human CXCL5 expression vector containing His tag, then treated with 600 ng/ml BFA for 3h, whole cell lysate with 5% NFDM/TBST
Lane 3: HEK-293T cells transfected with a human CXCL6 expression vector containing His tag, then treated with 600 ng/ml BFA for 3h, whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Exposure time: 10s
![Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505), expandable thumbnail](https://content.abcam.com/products/images/cxcl5-cxcl6-antibody-ep13083-ab198505--western-blot-img429949.jpg "Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)")
Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)
The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID: 23922745).
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used a GAPDH loading control.
All lanes: Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/10000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) starved overnight, whole cell lysate at 15 µg with 5% NFDM/TBST
Lane 2: A549 (Human lung carcinoma epithelial cell) starved overnight, then treated with 10ng/ml TNF-a, 10nM TPA and 0.1% BSA for 24 hours, whole cell lysate at 15 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Exposure time: 10s
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