AB284855

Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CXCL5 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.

View Alternative Names

ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5

3 Images

Lab

Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

This data was developed using ab198505, the same antibody clone in a different buffer formulation.

CXCL5 + CXCL6 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell extract with ab198505 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab198505 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : A549 (Human lung carcinoma epithelial cell), whole cell lysate, 10µg

Lane 2 : A549 whole cell lysate with the ab198505 at 1/30 dilution

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198505 in A549 whole cell lysate

All lanes:

Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (<a href='/en-us/products/primary-antibodies/cxcl5-cxcl6-antibody-ep13083-ab198505'>ab198505</a>) at 1/1000 dilution

All lanes:

A549 (Human lung carcinoma epithelial cell), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 12 kDa

false

Exposure time: 93s

Lab

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

This data was developed using ab198505, the same antibody clone in a different buffer formulation.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID : 23922745).

ab181602 was used a GAPDH loading control.

All lanes:

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (<a href='/en-us/products/primary-antibodies/cxcl5-cxcl6-antibody-ep13083-ab198505'>ab198505</a>) at 1/10000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) starved overnight, whole cell lysate at 15 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) starved overnight, then treated with 10ng/ml TNF-a, 10nM TPA and 0.1% BSA for 24 hours, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

This data was developed using ab198505, the same antibody clone in a different buffer formulation.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID : 23922745).

ab181602 was used a GAPDH loading control.

All lanes:

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (<a href='/en-us/products/primary-antibodies/cxcl5-cxcl6-antibody-ep13083-ab198505'>ab198505</a>) at 1/10000 dilution

Lane 1:

HEK-293T cells transfected with an empty expression vector containing His tag, hole cell lysate

Lane 2:

HEK-293T cells transfected with a human CXCL5 expression vector containing His tag, then treated with 600 ng/ml BFA for 3h, whole cell lysate

Lane 3:

HEK-293T cells transfected with a human CXCL6 expression vector containing His tag, then treated with 600 ng/ml BFA for 3h, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

false

Exposure time: 10s

Unconjugated

Anti-CXCL5 + CXCL6 antibody [EP13083]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP13083

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab284855 is the carrier-free version of ab198505

The production method for this product has been changed from hybridoma to recombinant on 15th April 2024.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-X-C motif chemokine ligands 5 and 6 (CXCL5 and CXCL6) also known as ENA-78 and GCP-2 respectively are small proteins with a molecular mass of approximately 12 kDa. These chemokines belong to the CXC chemokine family and play key roles as signaling proteins. CXCL5 and CXCL6 are mainly expressed in neutrophils epithelial cells and to some extent in macrophages. When they bind to their receptor CXCR2 they induce chemotaxis guiding immune cells toward sites of inflammation or injury.

Biological function summary

They coordinate immune response by mediating neutrophil recruitment to inflammatory sites. CXCL5 and CXCL6 often work as a team binding CXCR2 and aiding in protective tissue responses. Their roles extend further as they partake in triggering angiogenesis the formation of new blood vessels under physiological or pathological conditions such as wound healing or cancer. These activities imply their involvement in complex cellular networks and interactions.

Pathways

The involvement of CXCL5 and CXCL6 arises prominently in the NF-kB pathway and the MAPK signaling pathway. These pathways integral to immune and inflammatory responses reflect the chemokines' interaction with proteins like IL-8 and TNF-alpha emphasizing their importance in host defense mechanisms and inflammatory signaling cascades. Their ability to engage with these pathways demonstrates an essential role in modulating the intensity and duration of inflammatory responses.

CXCL5 and CXCL6 relate to conditions such as cancer and rheumatoid arthritis. In cancer these chemokines can enhance tumor progression by increasing angiogenesis and attracting cells that promote tumor growth. Similarly in rheumatoid arthritis their overexpression correlates with joint inflammation and tissue damage. Proteins like VEGF-A connected to these chemokines further underline their association with pathological angiogenesis and inflammatory process regulation in disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

See full target information CXCL5

Additional targets

CXCL6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free

Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise