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Knockout Tested Rabbit Recombinant Monoclonal CXCL5 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (AB243097), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (AB243097), expandable thumbnail
  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (AB243097), expandable thumbnail
  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (AB243097), expandable thumbnail
  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (AB243097), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Additional Targets

CXCL6

Alternative names

ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CXCL5 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22310-196
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C-X-C motif chemokine ligands 5 and 6 (CXCL5 and CXCL6) also known as ENA-78 and GCP-2 respectively are small proteins with a molecular mass of approximately 12 kDa. These chemokines belong to the CXC chemokine family and play key roles as signaling proteins. CXCL5 and CXCL6 are mainly expressed in neutrophils epithelial cells and to some extent in macrophages. When they bind to their receptor CXCR2 they induce chemotaxis guiding immune cells toward sites of inflammation or injury.

Biological function summary

They coordinate immune response by mediating neutrophil recruitment to inflammatory sites. CXCL5 and CXCL6 often work as a team binding CXCR2 and aiding in protective tissue responses. Their roles extend further as they partake in triggering angiogenesis the formation of new blood vessels under physiological or pathological conditions such as wound healing or cancer. These activities imply their involvement in complex cellular networks and interactions.

Pathways

The involvement of CXCL5 and CXCL6 arises prominently in the NF-kB pathway and the MAPK signaling pathway. These pathways integral to immune and inflammatory responses reflect the chemokines' interaction with proteins like IL-8 and TNF-alpha emphasizing their importance in host defense mechanisms and inflammatory signaling cascades. Their ability to engage with these pathways demonstrates an essential role in modulating the intensity and duration of inflammatory responses.

Associated diseases and disorders

CXCL5 and CXCL6 relate to conditions such as cancer and rheumatoid arthritis. In cancer these chemokines can enhance tumor progression by increasing angiogenesis and attracting cells that promote tumor growth. Similarly in rheumatoid arthritis their overexpression correlates with joint inflammation and tissue damage. Proteins like VEGF-A connected to these chemokines further underline their association with pathological angiogenesis and inflammatory process regulation in disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry (Intracellular) - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line that was serum starved for 4h, then treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) (Red) / Untreated control (Green) labeling CXCL5 + CXCL6with ab243097 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/2000 dilution was used as the secondary antibody.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID: 9057843; 23922745).

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling CXCL5 + CXCL6 with ab243097 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) is observed. Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA treatment. (PMID: 9057843; 23922745).

  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097), expandable thumbnail

    Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID: 23922745).

    All lanes: Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/500 dilution

    Lane 1: A549 (human lung carcinoma cell line) starved overnight, whole cell lysate at 10 µg

    Lane 2: A549 was starved overnight, then treated with 10ng/ml TNF-a, 10nM Phorbol-12-myristate-13-acetate (PMA) and 0.1% BSA for 24 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 12 kDa

    Exposure time: 3min

  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097), expandable thumbnail

    Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/1000 dilution

    Lane 1: His-GST-tagged human CXCL5 recombinant protein (aa37-114) 10 ng

    Lane 2: Human CXCL6 recombinant protein (aa40-114) without tag 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 12 kDa

    Exposure time: 3s

  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097), expandable thumbnail

    Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    False colour image of Western blot: Anti-CXCL5 + CXCL6 antibody [EPR22310-196] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab243097 was shown to bind specifically to CXCL5 + CXCL6. A band was observed at 12 kDa in treated wild-type A549 cell lysates with no signal observed at this size in CXCL6 knockout cell line ab275838 (knockout cell lysate ab275812). To generate this image, wild-type and CXCL6 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/1000 dilution

    Lane 1: Wild-type A549 Untreated Control cell lysate at 20 µg

    Lane 2: Wild-type A549 Treated TNFa (10 ng/mL, 24 h) + PMA (10 nM, 24 h) cell lysate at 20 µg

    Lane 3: Untreated control at 20 µg

    Lane 4: Treated TNFa (10 ng/mL, 24 h) + PMA (10 nM, 24 h) cell lysate at 20 µg

    Lane 5: Human Lung cell lysate at 20 µg

    Lane 6: MOLT-4 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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