Anti-CXCL7/PBP antibody [EPR19780]

• IP

Supplier Data

Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] (AB206966)

NAP2 was immunoprecipitated from 0.35 mg of Human serum with ab206966 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab206966 at 1/500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human serum 10μg (Input).

Lane 2 : ab206966 IP in Human serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206966 in Human serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

The band at 50 kD is likely to be human IgG in input and rabbit IgG heavy chain in +ve and –ve lane, but this has not been tested.

All lanes:

Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)

Predicted band size: 14 kDa

false

• WB

Supplier Data

Western blot - Anti-CXCL7/PBP antibody [EPR19780] (AB206966)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 11 kDa

false

• WB

Supplier Data

Western blot - Anti-CXCL7/PBP antibody [EPR19780] (AB206966)

All lanes:

Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution

All lanes:

Human NAP2 active recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 11 kDa

false

Exposure time: 5s