AB242948

Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal CXCL9 antibody. Carrier free. Suitable for sELISA and reacts with Rat, Mouse samples.

View Alternative Names

Mig, Scyb9, Cxcl9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Protein m119, Small-inducible cytokine B9, MIG, MuMIG

4 Images

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

sELISA

Lab

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Interpolated concentration of native CXCL9 (MIG) was measured at different sample concentrations. Undiluted sample is as follows : rat serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee the same sensitivity level due to biological variation.

sELISA

Lab

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Interpolated concentration of native CXCL9 (MIG) was measured at different sample concentrations. Undiluted samples are as follows : stimulated NR8383 supernatant (treated with 100 ng/mL rat IFN gamma for 72 hours) 1% and mock unstimulated NR8383 supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Interpolated concentrations of CXCL9 (MIG) in mock unstimulated NR8383 supernatant were below the detectable range of the assay. Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee the same sensitivity level due to biological variation.

sELISA

Lab

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Sandwich ELISA with the capture antibody ab242948 dilution at 2 μg/mL and detector antibody ab242976 dilution at 0.5 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab203364).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17005-14

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

ab242948 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MIG. The recommended pair for sandwich ELISA is:
Capture: ab242948, Mouse MIG Capture Antibody (unconjugated)
Detector: ab242976, Mouse MIG Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.

Biological function summary

This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.

Pathways

CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.

CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.

See full target information Cxcl9

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Sandwich ELISA - Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) (AB242948)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise