Rabbit Recombinant Monoclonal CXCL9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.
Images
![Western blot - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--western-blot-img435538.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435589.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435588.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435590.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (AB320828)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Mouse | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
Alternative names
Mig, Scyb9, Cxcl9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Protein m119, Small-inducible cytokine B9, MIG, MuMIG
Recommended products
Rabbit Recombinant Monoclonal CXCL9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28968-26
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab320828 is the carrier-free version of Anti-CXCL9 antibody [EPR28968-26] ab320827.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.
- Biological function summary
This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.
- Pathways
CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.
- Associated diseases and disorders
CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--western-blot-img435538.jpg "Western blot - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)")
Western blot - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)
This data was developed using Anti-CXCL9 antibody [EPR28968-26] ab320827, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:2115167).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CXCL9 antibody [EPR28968-26] (Anti-CXCL9 antibody [EPR28968-26] ab320827) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 30 µg
Lane 2: RAW 264.7 treated with 10ng/ml IFN gamma and 300ng/ml Brefeldin A/BFA for 8 hours whole cell lysate at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 17 kDa, 36 kDa
Exposure time: 81s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435589.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)
This data was developed using Anti-CXCL9 antibody [EPR28968-26] ab320827, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic cancer tissue labeling CXCL9 with Anti-CXCL9 antibody [EPR28968-26] ab320827 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of mouse pancreatic cancer.
The section was incubated with Anti-CXCL9 antibody [EPR28968-26] ab320827 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435588.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)
This data was developed using Anti-CXCL9 antibody [EPR28968-26] ab320827, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse Inflammatory bowel disease tissue labeling CXCL9 with Anti-CXCL9 antibody [EPR28968-26] ab320827 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining observed on immune cells in a mouse colon sample from an inflammatory bowel disease model (Dinitrobenzene sulfonic acid (DNBS)-induced model) (PMID: 34964822).
The section was incubated with Anti-CXCL9 antibody [EPR28968-26] ab320827 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828), expandable thumbnail](https://content.abcam.com/products/images/cxcl9-antibody-epr28968-26-bsa-and-azide-free-ab320828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435590.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR28968-26] - BSA and Azide free (ab320828)
This data was developed using Anti-CXCL9 antibody [EPR28968-26] ab320827, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling CXCL9 with Anti-CXCL9 antibody [EPR28968-26] ab320827 at 1/1000 (0.488 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse testis.
The section was incubated with Anti-CXCL9 antibody [EPR28968-26] ab320827 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com