Rabbit Recombinant Monoclonal CXCR1 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (AB273585), expandable thumbnail](https://content.abcam.com/products/images/cxcr1-antibody-epr23700-70-bsa-and-azide-free-ab273585--flow-cytometry-img105207.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (AB273585)")
![Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (AB273585), expandable thumbnail](https://content.abcam.com/products/images/cxcr1-antibody-epr23700-70-bsa-and-azide-free-ab273585--flow-cytometry-img440958.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (AB273585)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor (PubMed:1840701). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:8662698).
Alternative names
CD181, CMKAR1, IL8RA, CXCR1, C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, CDw128a, High affinity interleukin-8 receptor A, IL-8 receptor type 1, IL-8R A
Recommended products
Rabbit Recombinant Monoclonal CXCR1 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23700-70
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273585 is the carrier-free version of Anti-CXCR1 antibody [EPR23700-70] ab260008.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCR1 also known as IL-8 receptor A is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is primarily located on the surface of neutrophils a type of white blood cell and various other immune cells. CXCR1 recognizes and binds interleukin-8 (IL-8) an important chemokine that guides immune cells to sites of inflammation or infection. The binding of IL-8 to CXCR1 induces conformational changes activating intracellular signaling pathways which result in cellular responses like chemotaxis and phagocytosis.
- Biological function summary
CXCR1 plays an important role in the immune system's response to infection and inflammation. It binds to IL-8 as part of the body's inflammatory response recruiting neutrophils and other immune cells to infected or damaged tissues. This receptor is not known to be part of a larger protein complex but it interacts with various signaling molecules within the cell to modulate cellular responses. The proper functioning of CXCR1 is essential for a balanced immune response and effective pathogen clearance.
- Pathways
CXCR1 is an important component of the chemokine signaling pathway which is essential for the migration of immune cells to sites of inflammation. It also plays a critical role in the NF-kB signaling pathway which is involved in immune response and inflammation. Through these pathways CXCR1 interacts with other proteins like CXCR2 and IL-8 helping coordinate the movement and activation of immune cells necessary for defending against pathogens.
- Associated diseases and disorders
CXCR1 is linked to inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. It is involved in the excessive recruitment and activation of neutrophils contributing to tissue damage and disease progression. CXCR1 is also connected to CXCR2 in the context of these diseases as both receptors bind IL-8 and mediate similar inflammatory responses. Understanding CXCR1's role in these conditions can provide insights into developing targeted therapies aimed at reducing inflammation and disease severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (ab273585), expandable thumbnail](https://content.abcam.com/products/images/cxcr1-antibody-epr23700-70-bsa-and-azide-free-ab273585--flow-cytometry-img105207.jpg "Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (ab273585)")
Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (ab273585)
Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CXCR1 with Anti-CXCR1 antibody [EPR23700-70] ab260008 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).
A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or Anti-CXCR1 antibody [EPR23700-70] ab260008 (Right). Then stained with anti-CD56 conjugated to BV421.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR1 antibody [EPR23700-70] ab260008).
![Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (ab273585), expandable thumbnail](https://content.abcam.com/products/images/cxcr1-antibody-epr23700-70-bsa-and-azide-free-ab273585--flow-cytometry-img440958.jpg "Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (ab273585)")
Flow Cytometry - Anti-CXCR1 antibody [EPR23700-70] - BSA and Azide free (ab273585)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR1 antibody [EPR23700-70] ab260008).
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with Anti-CXCR1 antibody [EPR23700-70] ab260008 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-CXCR1 antibody [EPR23700-70] ab260008 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 0.2 μg/ml (1/10)) for 30min on ice. The cells were simultaneously stained with CD56.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com