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AB325848

Anti-CXCR1 antibody [HL2674] - BSA and Azide free

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Rabbit Recombinant Monoclonal CXCR1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Transfected cell lysate - Human samples. Immunogen corresponding to Synthetic Peptide within Human CXCR1.

View Alternative Names

CD181, CMKAR1, IL8RA, CXCR1, C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, CDw128a, High affinity interleukin-8 receptor A, IL-8 receptor type 1, IL-8R A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (AB325848)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (AB325848)

ab325848 detects CXCR1 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded rat liver. CXCR1 stained by ab325848 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (AB325848)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (AB325848)

ab325848 detects CXCR1 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded mouse liver. CXCR1 stained by ab325848 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (AB325848)
  • WB

Supplier Data

Western blot - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (AB325848)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325848 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR1 antibody [HL2674] - BSA and Azide free (ab325848) at 1/5000 dilution

Lanes 1, 3, 5, 7, 9, 11, 13 and 15:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled CXCR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled CXCR2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled CXCR4 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Unboiled CXCR5 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled CXCR6 transfected (+) 293T whole cell extracts at 30 µg

Lane 12:

Unboiled CXCR7 transfected (+) 293T whole cell extracts at 30 µg

Lane 14:

Unboiled CX3CR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 16:

Unboiled ACKR4 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2674

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CXCR1.

P25024

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor (PubMed : 1840701). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 8662698).
See full target information CXCR1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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