AB89251

Anti-CXCR1 antibody [MM0221-7D22]

Name: Anti-CXCR1 antibody [MM0221-7D22] (ab89251) | Abcam
Brand: Abcam Limited
SKU: AB89251
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CXCR1 antibody. Carrier free. Suitable for Flow Cyt, WB, FuncS (Neut/Block), IHC-Fr and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCR1.

View Alternative Names

CD181, CMKAR1, IL8RA, CXCR1, C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, CDw128a, High affinity interleukin-8 receptor A, IL-8 receptor type 1, IL-8R A

1 Images

Supplier Data

Western blot - Anti-CXCR1 antibody [MM0221-7D22] (AB89251)

All lanes:

Western blot - Anti-CXCR1 antibody [MM0221-7D22] (ab89251) at 1/500 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0221-7D22

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

FuncS (Neut/Block), WB, IHC-Fr, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CXCR1.

P25024

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR1 also known as IL-8 receptor A is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is primarily located on the surface of neutrophils a type of white blood cell and various other immune cells. CXCR1 recognizes and binds interleukin-8 (IL-8) an important chemokine that guides immune cells to sites of inflammation or infection. The binding of IL-8 to CXCR1 induces conformational changes activating intracellular signaling pathways which result in cellular responses like chemotaxis and phagocytosis.

Biological function summary

CXCR1 plays an important role in the immune system's response to infection and inflammation. It binds to IL-8 as part of the body's inflammatory response recruiting neutrophils and other immune cells to infected or damaged tissues. This receptor is not known to be part of a larger protein complex but it interacts with various signaling molecules within the cell to modulate cellular responses. The proper functioning of CXCR1 is essential for a balanced immune response and effective pathogen clearance.

Pathways

CXCR1 is an important component of the chemokine signaling pathway which is essential for the migration of immune cells to sites of inflammation. It also plays a critical role in the NF-kB signaling pathway which is involved in immune response and inflammation. Through these pathways CXCR1 interacts with other proteins like CXCR2 and IL-8 helping coordinate the movement and activation of immune cells necessary for defending against pathogens.

CXCR1 is linked to inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. It is involved in the excessive recruitment and activation of neutrophils contributing to tissue damage and disease progression. CXCR1 is also connected to CXCR2 in the context of these diseases as both receptors bind IL-8 and mediate similar inflammatory responses. Understanding CXCR1's role in these conditions can provide insights into developing targeted therapies aimed at reducing inflammation and disease severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor (PubMed : 1840701). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 8662698).

See full target information CXCR1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:318 PubMed39639338

2024

Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas A Horn,Hanne Lind,Kristen Fousek,Haiyan Qin,Nika Rajabian,Shantel Angstadt,Nicole Hsiao-Sanchez,Miriam M Medina-Enriquez,Marcus D Kelly,Clint T Allen,Sarah M Hammoudeh,Roberto Weigert,Dean Y Maeda,John A Zebala,Claudia Palena

Biochemical genetics 63:698-712 PubMed38509423

2024

Design of an In Vitro Model for Epithelial-to-Mesenchymal Transition in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhui Zhang,Ling Bi,Quanyao Li,Liqiu Yao,Xiao Wang,Hui Liu,Jun Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR1 antibody [MM0221-7D22]

Western blot - Anti-CXCR1 antibody [MM0221-7D22] (AB89251)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise