Rabbit Recombinant Multiclonal CXCR1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CXCR1.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (AB313462), expandable thumbnail](https://content.abcam.com/products/images/cxcr1-antibody-rp23040161-ab313462--immunocytochemistry-immunofluorescence-img417871.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (AB313462)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogens
This product was produced with the following immunogens:
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Target data
Function
Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor (PubMed:1840701). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:8662698).
Alternative names
CD181, CMKAR1, IL8RA, CXCR1, C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, CDw128a, High affinity interleukin-8 receptor A, IL-8 receptor type 1, IL-8R A
Recommended products
Rabbit Recombinant Multiclonal CXCR1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CXCR1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
This product was produced with the following immunogens:
- Clone number
- RP23040161
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCR1 also known as IL-8 receptor A is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is primarily located on the surface of neutrophils a type of white blood cell and various other immune cells. CXCR1 recognizes and binds interleukin-8 (IL-8) an important chemokine that guides immune cells to sites of inflammation or infection. The binding of IL-8 to CXCR1 induces conformational changes activating intracellular signaling pathways which result in cellular responses like chemotaxis and phagocytosis.
- Biological function summary
CXCR1 plays an important role in the immune system's response to infection and inflammation. It binds to IL-8 as part of the body's inflammatory response recruiting neutrophils and other immune cells to infected or damaged tissues. This receptor is not known to be part of a larger protein complex but it interacts with various signaling molecules within the cell to modulate cellular responses. The proper functioning of CXCR1 is essential for a balanced immune response and effective pathogen clearance.
- Pathways
CXCR1 is an important component of the chemokine signaling pathway which is essential for the migration of immune cells to sites of inflammation. It also plays a critical role in the NF-kB signaling pathway which is involved in immune response and inflammation. Through these pathways CXCR1 interacts with other proteins like CXCR2 and IL-8 helping coordinate the movement and activation of immune cells necessary for defending against pathogens.
- Associated diseases and disorders
CXCR1 is linked to inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. It is involved in the excessive recruitment and activation of neutrophils contributing to tissue damage and disease progression. CXCR1 is also connected to CXCR2 in the context of these diseases as both receptors bind IL-8 and mediate similar inflammatory responses. Understanding CXCR1's role in these conditions can provide insights into developing targeted therapies aimed at reducing inflammation and disease severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (ab313462), expandable thumbnail](https://content.abcam.com/products/images/cxcr1-antibody-rp23040161-ab313462--immunocytochemistry-immunofluorescence-img417871.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (ab313462)")
Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (ab313462)
For immunofluorescence analysis, HL-60 cells were fixed and permeabilized for detection of endogenous CXCR1 using ab313462 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1:2000). Panel a) shows representative cells that were stained for detection and localization of CXCR1 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of CXCR1. Panel e)represents control cells with no primary antibody to assess background Panel f) shows no signal in MDA-MB-231 cells which is negative for CXCR1 expression (Muller et. al., 2001, Nature). The images were captured at 60X magnification
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