AB313462

Anti-CXCR1 antibody [RP23040161]

Recombinant
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(1 Publication)

Rabbit Recombinant Multiclonal CXCR1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CXCR1.

View Alternative Names

CD181, CMKAR1, IL8RA, CXCR1, C-X-C chemokine receptor type 1, CXC-R1, CXCR-1, CDw128a, High affinity interleukin-8 receptor A, IL-8 receptor type 1, IL-8R A

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (AB313462)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (AB313462)

For immunofluorescence analysis, HL-60 cells were fixed and permeabilized for detection of endogenous CXCR1 using ab313462 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1 : 2000). Panel a) shows representative cells that were stained for detection and localization of CXCR1 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of CXCR1. Panel e)represents control cells with no primary antibody to assess background Panel f) shows no signal in MDA-MB-231 cells which is negative for CXCR1 expression (Muller et. al., 2001, Nature). The images were captured at 60X magnification

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040161

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human CXCR1. Database link P25024

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR1 also known as IL-8 receptor A is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is primarily located on the surface of neutrophils a type of white blood cell and various other immune cells. CXCR1 recognizes and binds interleukin-8 (IL-8) an important chemokine that guides immune cells to sites of inflammation or infection. The binding of IL-8 to CXCR1 induces conformational changes activating intracellular signaling pathways which result in cellular responses like chemotaxis and phagocytosis.

Biological function summary

CXCR1 plays an important role in the immune system's response to infection and inflammation. It binds to IL-8 as part of the body's inflammatory response recruiting neutrophils and other immune cells to infected or damaged tissues. This receptor is not known to be part of a larger protein complex but it interacts with various signaling molecules within the cell to modulate cellular responses. The proper functioning of CXCR1 is essential for a balanced immune response and effective pathogen clearance.

Pathways

CXCR1 is an important component of the chemokine signaling pathway which is essential for the migration of immune cells to sites of inflammation. It also plays a critical role in the NF-kB signaling pathway which is involved in immune response and inflammation. Through these pathways CXCR1 interacts with other proteins like CXCR2 and IL-8 helping coordinate the movement and activation of immune cells necessary for defending against pathogens.

CXCR1 is linked to inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. It is involved in the excessive recruitment and activation of neutrophils contributing to tissue damage and disease progression. CXCR1 is also connected to CXCR2 in the context of these diseases as both receptors bind IL-8 and mediate similar inflammatory responses. Understanding CXCR1's role in these conditions can provide insights into developing targeted therapies aimed at reducing inflammation and disease severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor (PubMed : 1840701). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 8662698).

See full target information CXCR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e12427 PubMed38545803

2024

The small extracellular vesicle-mediated intercellular transformation of CXCR1 to CXCR1 tumour cells promotes the progression of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoshi Xu,Aoming Cheng,Bo Li,Xutengyue Tian,Zhengxue Han,Zhien Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR1 antibody [RP23040161]

Immunocytochemistry/ Immunofluorescence - Anti-CXCR1 Antibody [RP23040161] (AB313462)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise