AB225732

Anti-CXCR2 antibody [EPR22301-103]

Name: Anti-CXCR2 antibody [EPR22301-103] (ab225732) | Abcam
Brand: Abcam Limited
SKU: AB225732
Price: 225 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal CXCR2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications.

View Alternative Names

CD182, IL8RB, CXCR2, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8 receptor type 2, IL-8R B

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of pancreas tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining inn stromal cells of human adenocarcinoma of pancreas (PMID : 27265504) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in stromal cells of human gastric adenocarcinoma (PMID : 28575019) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control : No staining on human cerebrum (PMID : 28529637) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in neutrophils of human spleen (PMID : 29661142) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Carrier free

Anti-CXCR2 antibody [EPR22301-103] - BSA and Azide free
660 APC

APC Anti-CXCR2 antibody [EPR22301-103]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CXCR2 antibody [EPR22301-103]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CXCR2 antibody [EPR22301-103]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CXCR2 antibody [EPR22301-103]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CXCR2 antibody [EPR22301-103]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CXCR2 antibody [EPR22301-103]
578 PE

PE Anti-CXCR2 antibody [EPR22301-103]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22301-103

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR2 also called C-X-C chemokine receptor type 2 is a G protein-coupled receptor with a mass of approximately 53 kDa. It primarily binds to chemokines like CXCL1 CXCL2 CXCL3 CXCL5 CXCL6 CXCL7 and CXCL8 (also known as IL-8). CXCR2 is expressed in a variety of cells but is found especially in neutrophils where it plays a role in mediating immune response. Additionally it shows expression in some epithelial and endothelial cells contributing to its specialized functions in various tissues.

Biological function summary

CXCR2 plays significant roles in mediating inflammatory responses and is part of the chemokine receptor family. It functions by promoting chemotaxis of neutrophils to sites of inflammation infection or injury. This receptor operates with other chemokine receptors in the recruitment and migration of different immune cells helping coordinate an effective immune response. CXCR2 does not function alone and often interacts with other cell surface receptors and signaling molecules during immune responses.

Pathways

CXCR2 contributes prominently to the chemokine signaling pathway and the inflammatory response pathway. It interacts closely with proteins like CXCR1 another chemokine receptor to perform its functions. These interactions can activate downstream signaling cascades involving MAPK and PI3K pathways which are important for various cellular responses like proliferation survival and migration.

CXCR2 has associations with inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In these conditions excessive activation or expression of CXCR2 can lead to pathological inflammation. CXCR2 coordinates with proteins like IL-8 contributing to the chemotaxis of inflammatory cells in affected tissues and this coordination often exacerbates the inflammatory process in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor (PubMed : 1891716). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 8662698). Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2 (PubMed : 1891716). Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues. Signals intravascular neutrophil chemotaxis to the injury site.

See full target information CXCR2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Hepatology research : the official journal of the Japan Society of Hepatology 55:1296-1306 PubMed40515742

2025

CXCL6 Is a Novel Biliary Marker and a Downstream Target of MMP7 in Biliary Atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Fanyang Kong,Jingying Jiang,Min Du,Rui Dong,Gong Chen,Shan Zheng,Junfeng Wang

Nature communications 15:10736 PubMed39737994

2024

Integrated multi-omics profiling reveals neutrophil extracellular traps potentiate Aortic dissection progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Fei Zhao,Zi-Ang Zuo,Zhe-Yun Li,Ye Yuan,Shi-Chai Hong,Wei-Guo Fu,Bin Zhou,Li-Xin Wang

Journal of clinical periodontology 51:196-208 PubMed38088448

2023

Single-cell sequencing identifies inflammation-promoting fibroblast-neutrophil interaction in peri-implantitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ji Mo,Yi-Rao Lai,Qian-Ru Huang,Yin-Ran Li,Yi-Jie Zhang,Rui-Ying Chen,Shu-Jiao Qian

Cell research 33:585-603 PubMed37337030

2023

An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wu,Jiayan Yan,Yinqi Bai,Feiyu Chen,Xuanxuan Zou,Jiangshan Xu,Ao Huang,Liangzhen Hou,Yu Zhong,Zehua Jing,Qichao Yu,Xiaorui Zhou,Zhifeng Jiang,Chunqing Wang,Mengnan Cheng,Yuan Ji,Yingyong Hou,Rongkui Luo,Qinqin Li,Liang Wu,Jianwen Cheng,Pengxiang Wang,Dezhen Guo,Waidong Huang,Junjie Lei,Shang Liu,Yizhen Yan,Yiling Chen,Sha Liao,Yuxiang Li,Haixiang Sun,Na Yao,Xiangyu Zhang,Shiyu Zhang,Xi Chen,Yang Yu,Yao Li,Fengming Liu,Zheng Wang,Shaolai Zhou,Huanming Yang,Shuang Yang,Xun Xu,Longqi Liu,Qiang Gao,Zhaoyou Tang,Xiangdong Wang,Jian Wang,Jia Fan,Shiping Liu,Xinrong Yang,Ao Chen,Jian Zhou

Translational oncology 27:101568 PubMed36270103

2022

Single-cell RNA sequencing analysis revealed cellular and molecular immune profiles in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hao,Ziyao Zhang,Zilong Lu,Juan Xiong,Tao Fan,Congkuan Song,Ruyuan He,Lin Zhang,Shize Pan,Donghang Li,Heng Meng,Weichen Lin,Bin Luo,Jinfeng Yang,Ning Li,Qing Geng

Cell death & disease 13:624 PubMed35853880

2022

Targeting HIC1/TGF-β axis-shaped prostate cancer microenvironment restrains its progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqi Wu,Wenfeng Wang,Guohai Shi,Mingang Hao,Yingying Wang,Mengfei Yao,Yongqiang Huang,Leilei Du,Xingming Zhang,Dingwei Ye,Xiaojie Bian,Jianhua Wang

Journal of nanobiotechnology 20:218 PubMed35525963

2022

Targeted neutrophil-mimetic liposomes promote cardiac repair by adsorbing proinflammatory cytokines and regulating the immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Yanan Song,Qiaozi Wang,Qiyu Li,Haipeng Tan,Jinfeng Gao,Ning Zhang,Xueyi Weng,Dili Sun,Wusiman Yakufu,Zhengmin Wang,Juying Qian,Zhiqing Pang,Zheyong Huang,Junbo Ge

Nature communications 13:1588 PubMed35332119

2022

Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Peitao Zhang,Haixia Guan,Shukai Yuan,Huili Cheng,Jian Zheng,Zhenlei Zhang,Yifan Liu,Yang Yu,Zhaowei Meng,Xiangqian Zheng,Li Zhao

Phytomedicine : international journal of phytotherapy and phytopharmacology 96:153908 PubMed35026516

2022

Astragaloside IV promotes the angiogenic capacity of adipose-derived mesenchymal stem cells in a hindlimb ischemia model by FAK phosphorylation via CXCR2.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyi Wang,Zekun Shen,Yanan Tang,Bingyi Chen,Jinxing Chen,Jiaxuan Hou,Jiayan Li,Mengzhao Zhang,Shuang Liu,Yifan Mei,Liwei Zhang,Shaoying Lu

Technology in cancer research & treatment 19:1533033820957026 PubMed33089764

2020

Circ_0000215 Increases the Expression of CXCR2 and Promoted the Progression of Glioma Cells by Sponging miR-495-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Nurehemaiti Mutalifu,Peng Du,Jingjing Zhang,Halik Akbar,Baofeng Yan,Sulaiman Alimu,Lingxiao Tong,Xinping Luan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR2 antibody [EPR22301-103]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise