Anti-CXCR2 antibody [HL2604] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

ab325849 detects CXCR2 protein at cell membrane by immunofluorescent analysis. Sample : THP-1 cells were fixed in ice-cold MeOH for 5 min. Green : CXCR2 stained by ab325849 diluted at 1 : 200 and competitor's antibody diluted at 1 : 200. Blue : Fluoroshield with DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

ab325849 detects CXCR2 protein by immunofluorescent analysis. Sample : THP-1 cells were fixed in ice-cold MeOH for 5 min. Green : CXCR2 stained by ab325849 diluted at 1 : 100. Blue : Fluoroshield with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

ab325849 detects CXCR2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded human pancreatic cancer. CXCR2 stained by ab325849 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

Untreated (–) and treated (+) unboiled THP-1 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325849 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (ab325849) at 1/1000 dilution

Lane 1:

Untreated (–) unboiled THP-1 whole cell extract at 30 µg

Lane 2:

PNGase F treated (+) unboiled THP-1 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

Unboiled THP-1 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325849 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (ab325849) at 1/1000 dilution

Lane 1:

Unboiled THP-1 whole cell extracts at 30 µg

Lane 2:

Unboiled THP-1 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325849 diluted at 1 : 1000 and the competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (ab325849) at 1/1000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged CXCR2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged CXCR2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325849 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (ab325849) at 1/5000 dilution

Lanes 1, 3, 5, 7, 9, 11, 13 and 15:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled CXCR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled CXCR2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled CXCR4 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Unboiled CXCR5 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled CXCR6 transfected (+) 293T whole cell extracts at 30 µg

Lane 12:

Unboiled CXCR7 transfected (+) 293T whole cell extracts at 30 µg

Lane 14:

Unboiled CX3CR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 16:

Unboiled ACKR4 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325849 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (ab325849) at 1/1000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged CXCR2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged CXCR2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• Array

Supplier Data

Array - Anti-CXCR2 antibody [HL2604] - BSA and Azide free (AB325849)

ab325849 specifically detects CXCR2 protein on a VirDTM-GPCR array. Array was probed with antibody using a 1 : 1000 dilution. Yellow : CXCR2 . Blue : CXCR1, CXCR3, CXCR4, CXCR5, or CXCR6. White : Positive control.