AB61100

Anti-CXCR2 (phospho S347) antibody

Name: Anti-CXCR2 (phospho S347) antibody (ab61100) | Abcam
Brand: Abcam Limited
SKU: AB61100

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(3 Publications)

Rabbit Polyclonal CXCR2 phospho S347 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CXCR2 pS347 aa 300 to C-terminus.

View Alternative Names

CD182, IL8RB, CXCR2, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8 receptor type 2, IL-8R B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 (phospho S347) antibody (AB61100)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 (phospho S347) antibody (AB61100)

1/50-1/100 ab61100 staining phosphorilated CXCR2 on human breast carcinoma tissue, in the presence or absence of the immunizing phospopeptide.

Supplier Data

Western blot - Anti-CXCR2 (phospho S347) antibody (AB61100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-IL-8Rβ (Phospho-Ser347) antibody.

All lanes were loaded with lysates from PMA-stimulated 3T3 cells.

Lane 2 : The membrane was incubated with anti-IL-8Rβ (Phospho-Ser347) antibody in the presence of phospho-IL-8Rβ peptide (blocking peptide).

All lanes:

Western blot - Anti-CXCR2 (phospho S347) antibody (ab61100) at 1/500 dilution

All lanes:

PMA-stimulated 3T3 cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody at 1/5000 dilution

Predicted band size: 41 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CXCR2 pS347 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25025

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR2 also called C-X-C chemokine receptor type 2 is a G protein-coupled receptor with a mass of approximately 53 kDa. It primarily binds to chemokines like CXCL1 CXCL2 CXCL3 CXCL5 CXCL6 CXCL7 and CXCL8 (also known as IL-8). CXCR2 is expressed in a variety of cells but is found especially in neutrophils where it plays a role in mediating immune response. Additionally it shows expression in some epithelial and endothelial cells contributing to its specialized functions in various tissues.

Biological function summary

CXCR2 plays significant roles in mediating inflammatory responses and is part of the chemokine receptor family. It functions by promoting chemotaxis of neutrophils to sites of inflammation infection or injury. This receptor operates with other chemokine receptors in the recruitment and migration of different immune cells helping coordinate an effective immune response. CXCR2 does not function alone and often interacts with other cell surface receptors and signaling molecules during immune responses.

Pathways

CXCR2 contributes prominently to the chemokine signaling pathway and the inflammatory response pathway. It interacts closely with proteins like CXCR1 another chemokine receptor to perform its functions. These interactions can activate downstream signaling cascades involving MAPK and PI3K pathways which are important for various cellular responses like proliferation survival and migration.

CXCR2 has associations with inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In these conditions excessive activation or expression of CXCR2 can lead to pathological inflammation. CXCR2 coordinates with proteins like IL-8 contributing to the chemotaxis of inflammatory cells in affected tissues and this coordination often exacerbates the inflammatory process in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor (PubMed : 1891716). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 8662698). Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2 (PubMed : 1891716). Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues. Signals intravascular neutrophil chemotaxis to the injury site.

See full target information CXCR2 pS347

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:862675 PubMed35592244

2022

CXC Chemokine Receptor 2 Accelerates Tubular Cell Senescence and Renal Fibrosis β-Catenin-Induced Mitochondrial Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Meng,Jiewu Huang,Xian Ling,Shan Zhou,Jingyan Wei,Mingsheng Zhu,Jinhua Miao,Weiwei Shen,Jiemei Li,Huiyun Ye,Hongxin Niu,Yunfang Zhang,Lili Zhou

Journal of vascular research 56:39-53 PubMed30995642

2019

Electrical Stimulation Directs Migration, Enhances and Orients Cell Division and Upregulates the Chemokine Receptors CXCR4 and CXCR2 in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Filipa Cunha,Ann M Rajnicek,Colin D McCaig

Cancer letters 308:91-9 PubMed21601983

2011

Mesenchymal stem cells promote mammary cancer cell migration in vitro via the CXCR2 receptor.

Applications

ICC/IF, WB

Species

Mouse, Mouse

Jennifer L Halpern,Amy Kilbarger,Conor C Lynch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CXCR2 (phospho S347) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 (phospho S347) antibody (AB61100)

Western blot - Anti-CXCR2 (phospho S347) antibody (AB61100)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise