AB259865

Anti-CXCR3 antibody [EPR23845-44]

20ul selling size
RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal CXCR3 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD183, GPR9, CXCR3, C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, CKR-L2, G protein-coupled receptor 9, Interferon-inducible protein 10 receptor, IP-10 receptor

2 Images

Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (AB259865)

Flow Cyt

Unknown

Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (AB259865)

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) (treated with 200U/ml Interleukin-2 (IL-2) and 1μg/ml Phytohemagglutinin (PHA) for 6 days) labelling CXCR3 with ab259865 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG or ab259865. Then stained with anti-CD3 conjugated to Alexa Fluor^® 647.

Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (AB259865)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) (top) or PBMCs treated with 5 µg/ml phytohaemagglutinin (PHA) for 48 hours (bottom), with ab259865 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab259865 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 µl at 1.0 μg/ml (1/2,000 dilution)) for 30 mins on ice. The cells were simultaneously stained with CD4.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 dilutionfor 30 mins on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

Carrier free

Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free
660 APC

APC Anti-CXCR3 antibody [EPR23845-44]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23845-44

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50500", "FlowCyt-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50500", "FlowCyt-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The chemokine receptor CXCR3 also known as CD183 is a protein that plays an important role in immune responses. This receptor is a part of the G protein-coupled receptor (GPCR) family and weighs approximately 41 kDa. CXCR3 is expressed on activated T cells some B cells natural killer (NK) cells and certain subsets of dendritic cells. Its expression is mostly found in inflamed tissues and in conditions where the immune system is active such as infections or autoimmune diseases.

Biological function summary

CXCR3 is involved in directing the migration of immune cells to sites of inflammation. This receptor binds to specific chemokines—CXCL9 CXCL10 and CXCL11—that are produced in response to cytokines like IFN-gamma. CXCR3 does not function as part of a larger protein complex; instead it operates independently facilitating the trafficking and activation of its expressing cells. Its activity is pivotal in immune responses assisting in rapid mobilization and targeting of effector cells to needed areas.

Pathways

CXCR3 is a component of the chemokine signaling pathway which is important for leukocyte chemotaxis. Within this pathway CXCR3 interacts with other receptors and proteins that modulate immune responses such as CXCR4 and CCR5 which also mediate chemotactic migration of leukocytes. Moreover it plays a role in the Jak-STAT signaling pathway impacting gene transcription and cell survival particularly in response to inflammatory signals.

CXCR3 is closely associated with autoimmune diseases like multiple sclerosis and rheumatoid arthritis due to its role in inflammation and immune cell recruitment. The receptor is also involved in cancer where its dysregulation can impact tumor growth and metastasis. CXCR3 through its interaction with chemokines like CXCL10 can influence the tumor microenvironment by affecting the infiltration and function of immune cells. Understanding these interactions can provide insights into therapeutic strategies targeting CXCR3 in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed : 12782716). Binds to CCL21. Probably promotes cell chemotaxis response. Upon activation by PF4, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling.. Isoform 2. Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway (PubMed : 12782716). Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis.. Isoform 3. Mediates the activity of CXCL11.

See full target information CXCR3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e723 PubMed36301026

2022

Low serum level of 25-OH vitamin D relates to Th17 and treg changes in colorectal cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Bai Chen,Liugen Jin

Aging 14:1910-1931 PubMed35210369

2022

Expression profile and prognostic values of Chromobox family members in human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juanni Li,Zhijie Xu,Lei Zhou,Kuan Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR3 antibody [EPR23845-44]

Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (AB259865)

Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] (AB259865)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise